Bacteriophage T7 Gene, promoter and RNAse List

Far Left Side of Genome

Class I genes - transition to phage metabolism

• E. coli promoter A0 (transcribes leftward)
• T7 promoter phiOL
• E. coli promoter A1
• E. coli promoter A2
• E. coli promoter A3
• RNase III site R0.3
• gene 0.3, inhibits EcoB and EcoK host restriction
• gene 0.4
• gene 0.5
• E. coli B promoter
• possible gene 0.6B
• gene 0.6A
• gene 0.7, protein kinase
• E. coliC promoter
• RNase III site R1
• gene 1, T7 RNA polymerase

Class II Genes - T7 replication

• T7 promoter phi1.1A
• RNase III site R1.1
• T7 promoter phi1.1B
• gene 1.1
• gene 1.2, inhibits host dGTP triphosphohydrolase/target for F plasmid exclusion
• T7 promoter phi1.3
• RNase III site R1.3
• E. coli transcription terminator TE
• gene 1.4
• T7 promoter phi1.5
• gene 1.5
• T7 promoter phi1.6
• gene 1.6
• gene 1.7
• gene 1.8
• gene 2, inhibits E. coli RNA polymerase
• T7 promoter phi2.5
• gene 2.5, single-stranded DNA-binding protein
• gene 2.8
• gene 3, endonuclease
• gene 3.5, lysozyme/inhibits T7 RNA polymerase [7]
• T7 promoter phi3.8
• possible RNase III site R3.8
• gene 3.8
• gene 4A, primase/helicase [14,15]
• gene 4.1
• gene 4B/helicase
• T7 promoter phi4c
• gene 4.2
• T7 promoter phi4.3
• gene 4.3
• gene 4.5
• RNase III site R4.7
• T7 promoter phi4.7
• gene 4.7
• gene 5, DNA polymerase
• gene 5.3
• gene 5.5, permits growth on lambda lysogens /binds host nucleoid protein H-NS
• possible gene 5.5-5.7
• gene 5.7
• gene 5.9, inhibits host recBCD nuclease [13]
• gene 6, exonuclease
• gene 6.3

Class III genes - phage particle proteins, packaging and maturation of phage

• T7 promoter phi6.5
• RNase III site R6.5
• gene 6.5
• gene 6.7
• gene 7, host range
• gene 7.3, host range
• gene 7.7
• gene 8, head-tail connector protein
• T7 promoter phi9
• gene 9, scaffolding protein
• T7 promoter phi10
• gene 10B, minor capsid protein [12]
• gene 10A, major capsid protein
• T7 transcription terminator Tphi
• gene 11, tail protein
• gene 12, tail protein
• possible RNase III site R13
• gene 13, internal virion protein
• gene 14, internal virion protein
• gene 15, internal virion protein
• gene 16, internal virion protein
• T7 promoter phi17
• gene 17, tail fiber protein
• gene 17.5, lysis protein
• gene 18, DNA maturation protein
• E. coli promoter E
• RNase III site R18.5
• gene 18.5, homologous to lambda Rz lysis protein
• gene 18.7
• gene 19, DNA maturation protein
• gene 19.2
• gene 19.3
• T7 promoter phiOR
• gene 19.5

Far Right Side of Genome