H1-gamma

Function

H1-gamma is a gene encoding the late histone subtype.
The H1-gamma gene appears to be a unique sequence gene that is not tightly linked to the core histone genes.
This gene is present in a single copy per haploid genome and encodes an mRNA of 752 nucleotides (Knowles et al.,1987).

Protein

H1-gamma belongs to the late histone subtype.
The primary structure of this protein is 209 amino acids in length.
It has the tripartite structure (nose, head and tail) typical of this class of histones. The head regions (amino acids 17 to 85) are believed to be globular domains. The 3` tail region of H1-gamma is extremely basic, consisting almost exclusively of lysine, alanine, and proline. H1-gamma contains no serine residues in the C-terminal tail. The last 66 amino acids of H1-gamma are composed almost entirely of two repeated pentapeptides. The peptide PAAKK is repeated four times between amino acids 151 and 171, and the related peptide KPAKK is repeated five times from amino acid 177 to the C-terminus.
The large nomber of lysine residues gives H1-gamma a net positive charge of 70 (Knowles et al., 1986).
SWISS_PROT: P07796

Subcellular location

Nuclear

Expression Pattern

There are 3,900 stored maternal H1-gamma mRNA transcripts per egg.
The number of H1-gamma transcripts per embryo rises by 9.5 h postfertilization, but the maximum rate of accumulation (4,300 molecules per min per embryo) occurs in the late-blastula-stage embryo between 14 and 21 h after fertilization. The number of H1-gamma mRNA molecules peaks 21 h after fertilization when there are 2.0 X 10(6) molecules per embryo (a 500-fold increase) and then decreases over the next 3.25 h to 1.3 million molecules per embryo.
Between 24 and 82 h after fertilization the number of H1-gamma transcripts declines steadily (210 molecules per min per embryo) to reach approximately 5.4 X 10(5) H1-gamma mRNAs by 82 h postfertilization (Knowles et al.,1987).

The relative amounts of the H1-gamma mRNA during development
Method: Nothern blot hybridization.
Reference: Knowles et al., 1987.

Stage Egg 13 hr 15 hr 21 hr 24 hr 40 hr 50 hr 82 hr

Level 3.900 9*10^4 1.5*10^5 2*10^6 1.3*10^6 1.1*10^6 6.1*10^5 5.4*10^5