Lvmsp130

Function

Lvmsp130 is a PMC marker gene. PMC-specific gene products participate in the synthesis of sceleton (Guss et al., 1997).
Msp130 is a sulfated cell-surface glycoprotein that has been implicated in calcium uptake or deposition (Carson et al., 1985; Leaf et al., 1987).

Protein

Subcellular location

Expression Pattern

Nothern blot analysis recognized the Lvmsp130 transcript of approximately 4kb, which is detectable by the hatched blastula stage. Expression levels peak at the mid gastrula stage and decrease through the late pluteus stage.
By whole-mount in situ hybridization, expression is detectable in the vegetal plate of hatched blastula-stage embryos, prior to PMC ingression. All PMCs in the embryo appear to express equal levels of the Lvmsp130 through the late gastrula stage. By the prism stage, however, a gradual reduction in transcript level begins in PMC throughout the embryo except those located in the ventrolateral clusters. By the pluteus larva stage, transcript levels are highest in cells at the tips of the elongating anterolateral and postoral rods, while intermediate levels of expression are visible in cells in the sheitel. At this stage the Lvmsp130 transcript is no longer detectable in cells distributed along the shafts of the sceletal rods (Guss et al., 1997).

mRNA level

Temporal accumulation (persent maximum abundance*)
Method 1: Nothern blot hybridization
Reference: Guss et al., 1997

Stage Hatched blastula Early mesenchyme blastula Late mesenchyme blastula Midgastrula Late gastrula Prism Early pluteus Late pluteus

Level 39 51 80 100 51 39 38 22

*Values were corrected for relative amounts of hybridizable RNA based on ubiquitin expression levels.

Spatial localization
Method 1: Whole-mount in situ hybridization
Reference: Guss et al., 1997

Stage Hatched blastula (prior to PMCs ingression) Late mesenchyme blastula Late gastrula Prism Early pluteus Late pluteus

Tissue vegetal plate all PMCs all PMCs PMCs in the ventrolateral clusters, particularly along the anonymous rods (highest level) PMCs at the tips of the budding postoral arms (highest level) PMCs at the tips of the postoral and anterolateral rods (highest level). Intermediate expression in the scheitel

Sequences

GenBank

Regulatory Regions

Regions

Regulatory Connections

Upstream Genes

Lvmsp130

Downstream Genes

Evolutionary Homologues

msp130 Strongylocentrotus purpuratus

Bibliography

UrchiNet

Search the GeNet

Stage	Hatched blastula	Early mesenchyme blastula	Late mesenchyme blastula	Midgastrula	Late gastrula	Prism	Early pluteus	Late pluteus
Level	39	51	80	100	51	39	38	22

Stage	Hatched blastula (prior to PMCs ingression)	Late mesenchyme blastula	Late gastrula	Prism	Early pluteus	Late pluteus
Tissue	vegetal plate	all PMCs	all PMCs	PMCs in the ventrolateral clusters, particularly along the anonymous rods (highest level)	PMCs at the tips of the budding postoral arms (highest level)	PMCs at the tips of the postoral and anterolateral rods (highest level). Intermediate expression in the scheitel

Lvmsp130

Function

Protein

Subcellular location

Expression Pattern

Sequences

Regulatory Regions

Regulatory Connections

Upstream Genes

Lvmsp130

Downstream Genes

Evolutionary Homologues

Links

Bibliography