Lytechinus
variegatus Genes in Development: Primary
mesenchyme-specific genes
LvSM50
Function
LvSM50 is a PMC marker gene. PMC-specific gene products
participate in the synthesis of sceleton (Guss et al., 1997).
SM50 was previously characterized as a basic protein of the spicule matrix. The specific role of matrix proteins have not
been elucidated, although they may function in the nucleation or orientation of crystal
growth (Sucov et al., 1987; Livingston et al., 1991; Katoh-Fukui et al., 1991, 1992; Harkey et al., 1995).
Protein
Subcellular location
Expression Pattern
The LvSM50 transcript is approximately 1.6 kb in length.
Although very faint signal is detectable at the hatched blastula stage
by nothern blot analysis, levels increase significantly at the mesenchyme
blastula stage. LvSM50 transcripts increase in abundance as development
proceed.
By whole-mount in situ hybridization, LvSM50 transcripts are first
detectable in PMCs at the mesenchyme blastula stage. This transcript
is initially expressed at approximately equal levels in all PMCs but shows
reproducible, regional variations in expression at later developmental stages.
Specifically, a distinct increase in abundance is exhibited by PMCs in the
scheitel by the late pluteus stage (Guss et al., 1997).
mRNA level
Temporal accumulation (persent maximum abundance*)
Method 1: Nothern blot hybridization
Reference: Guss et al., 1997
Stage
Hatched blastula
Early mesenchyme blastula
Late mesenchyme blastula
Midgastrula
Late gastrula
Prism
Early pluteus
Late pluteus
Level
0.1
2
20
30
27
37
41
100
*Values were corrected for relative amounts of hybridizable RNA
based on ubiquitin expression levels.
Spatial localization
Method 1: Whole-mount in situ hybridization
Reference: Guss et al., 1997
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