Strongylocentrotus purpuratus Genes in Development: Tektin
Tektin C1
Function
Tektin C1 is one of three integral filamentous protein components
of outer doublet microtubules. It is synthesized in sea urchins
in an amount correlating to the length of embryonic cilia.
The presence of low levels of tektin mRNAs at stages of
development prior to ciliogenesis may indicate that the tektins
are also involved in other cellular processes such as centriole replication,
mitosis and cell division (Norrander et al., 1995).
Protein
Tektin C1 is a ~ 47 KDa protein (Norrander et al., 1995).
GenBank: 3378105
Subcellular location
Expression Pattern
Nothern blotting showed that low levels of tektin C1 mRNA were detected in the
unfertilized egg, and there were no changes in message size observed
throughout development. Ciliogenesis in this species begins at about
8 hours, after which point there was a marked rise in the level
of each tektin mRNA. Post- fertilization levels of tektin B1 and tektin C1 mRNAs
were higher than those of tektin A1 mRNA.
With respect to peak levels, tektin C1 mRNA increased about 7-fold
over post-fertilization levels.
The message level fell rapidly after hatching, however the postciliogenesis level were
somewhat higher than those seen in the early cleavage stages (Norrander et al., 1995).
Tektins form filamentous polymers in the walls of ciliary and flagellar
microtubules. The organization of tektins in microtubules is not known in
detail, but tektins probably interact directly with tubulin.
Immunofluorescence microscopy has demonstrated that tektins A1, B1 and C1 are present in all nine flagellar doublet microtubules and possibly in the central pair microtubules (Steffen and Linck, 1988).
mRNA level
Temporal accumulation *
Method: Nothern blot analysis
Reference: Norrander et al., 1995
Stage
Unfertilized egg
3 hours
8 hours
12.5 hours (blastula)
16 h 50 min (rotating blastula)
18.75 hours (hatched, swimming blastula)
19 hours
24 h 50 min (mid-gastrula)
Level
0.1
0.15
0.2
0.41
0.92
1.0
0.75
0.34
* The autoradiograms were quantitated densitometrically,
normalizing mRNA band with corresponding ubiquitin signal.
Ectopic expression
Action of animalizing agents
To determine if tektin mRNA levels were increased in embryos
deploying cilia of increased ciliary length, normal embryos were compared with
zinc-animalized embryos.
At the period of ciliogenesis, when the tektin mRNA reached a peak,
the levels of tektin C1 mRNA were measured in normal and animalized embryos.
During this period, ciliary elongation was essentially linear and the level of tektin C1 mRNA remained relatively constant.
In the case of zinc-treated embryos the tektin C1 mRNA was up-regulated and its level increased 2.4 times (ubiquitin and tubulin levels increased 2.3 and ~2-fold respectively).
These levels do not correlate with the 1/3 ciliary length increase (Norrander et al., 1995).
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