LOCUS       SPSPEC11      640 bp    DNA             INV       04-APR-1995
DEFINITION  Strongylocentrotus purpuratus Spec1 gene exon 1 (for major
            calcium-binding protein).
ACCESSION   X03287
NID         g10315
KEYWORDS    calcium binding protein; spec1 gene.
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Hardin,S.H., Carpenter,C.D., Hardin,P.E., Bruskin,A.M. and
            Klein,W.H.
  TITLE     Structure of the Spec1 gene encoding a major calcium-binding
            protein in the embryonic ectoderm of the sea urchin,
            Strongylocentrotus purpuratus
  JOURNAL   J. Mol. Biol. 186 (2), 243-255 (1985)
  MEDLINE   86115267
COMMENT     see x03287 - x03292 for spec1 gene.
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
     promoter        347..355
                     /note="CAAT sequence"
     promoter        386..392
                     /note="TATA sequence"
     mRNA            415..634
                     /note="exon 1"
     misc_feature    415
                     /note="transcription initiation site"
     CDS             join(632..634,X03288:16..52,X03289:15..158,X03290:15..144,
                     X03291:15..130,X03292:16..44)
                     /note="calcium-binding protein (aa 1)"
                     /codon_start=1
                     /label=cbin_cds
                     /db_xref="PID:g763026"
                     /db_xref="SWISS-PROT:P04109"
                     /translation="MAAQLLFTDEEVTEFKRRFKNKDTDKSKSITAEELGEFFKSTGK
                     SYTDKQIDKMISDVDTDESGTIDFSEMLMGIAEQMVKWTWKEEHYTKAFDDMDKDGNG
                     SLSPQELREALSASKPPMKRKKIKAIIQKADANKDGKIDREEFMKLIKSC"
     intron          635..>640
                     /note="intron I (2.8 kb)"
BASE COUNT      189 a    146 c    114 g    191 t
ORIGIN
        1 gacattacga ccaatgaaac tttattttgg ttatcaaatg cttttttcgt gctcggatag
       61 ctctactgaa gacgaaacca tttcaaccag caaaatcgac caaggaaatc atgagttgga
      121 gcgaatgtaa ggttttcatc atcatgattt tctcatctaa ttattgacag tttccgaaaa
      181 tttccgaaat ttgtatatag cacggtcaca ccggcaaagc aaaagccgcc gtgctcacag
      241 gtgcatgcat gaataatgat tacataactt tcgtgagcat atatccagaa ttagtctcag
      301 tgggttttac aattttgttc aagtgacctt tatctgcgca tgcacagatc aatccgcgca
      361 tgctcagatt gtatctacga actggtatat ttgttagaac tgcaacccca cactacgcct
      421 aatacgcctt aaacatcagc aacgacattc ctagcgtatg gtcacgtgct ttatttcagg
      481 agttatcact ctttcttgaa taatactctt cgtcagcact ccacccttac cactccttga
      541 aagcaagtcg actagaagag aagtataggt acctgagctc cagaggaatt ttctatcttc
      601 aattttgccg gatctatccc aacttccaaa gatggtaagt
//