LOCUS SPU18132 2848 bp DNA INV 26-JAN-1996
DEFINITION Strongylocentrotus purpuratus 27 kDa primary mesenchyme-specific
spicule protein precursor PM27 gene, complete cds.
ACCESSION U18132
NID g603756
KEYWORDS .
SOURCE Sea urchin.
ORGANISM Strongylocentrotus purpuratus
Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
Strongylocentrotidae; Strongylocentrotus.
REFERENCE 1 (bases 1 to 2848)
AUTHORS Harkey,M.A., Klueg,K., Sheppard,P. and Raff,R.A.
TITLE Structure, expression, and extracellular targeting of PM27, a
skeletal protein associated specifically with growth of the sea
urchin larval spicule
JOURNAL Dev. Biol. 168 (2), 549-566 (1995)
MEDLINE 95246928
REFERENCE 2 (bases 1 to 2848)
AUTHORS Harkey,M.A.
TITLE Direct Submission
JOURNAL Submitted (06-DEC-1994) Michael A. Harkey, Dept. of Orthopaedics,
University of Washington, Seattle, WA 98195, USA
FEATURES Location/Qualifiers
source 1..2848
/organism="Strongylocentrotus purpuratus"
/db_xref="taxon:7668"
/clone="SpMG20-Xba-A"
/clone_lib="SpMG library of M.A. Harkey"
mRNA 1081..2420
sig_peptide 1207..1254
/note="predicted by von Heijne method"
CDS 1207..2010
/codon_start=1
/product="27 kDa primary mesenchyme-specific spicule
protein precursor PM27"
/db_xref="PID:g603757"
/translation="MKLLAILLVLPALCFGQRHEGPGMGPGMGPGMGPGMGPGMGPGM
GPGMGPGMGPGQGQGQGQGQGQVGGSKCKGGWFLIGQQCFKMMSRALKWNDAELMCEQ
NAPCGTPVLGGVMTIPDIQTSNAVINHLKSLSSTAMAIDIPFWTGLHNKWNALLERYE
GWKWPAGWSTTQQPLRFVNWAPREPNNQLLDQQHSYCARMNRMGQWYVVRCDEPMYFA
CSMPVSPPLVGGANTNPGMGMLVENPAPIINGYTEFESGLLMRNGVGGP"
mat_peptide 1255..2007
/product="27 kDa primary mesenchyme-specific spicule
protein PM27"
polyA_site 2420
BASE COUNT 840 a 527 c 646 g 835 t
ORIGIN
1 ctgcaggcga ctctagaaat gattgagaat gctgaataaa catgatttct attatggtat
61 tttagaatca tcaaaacgaa acgatgtttc ctttgtaaag caatgtacac acaaatatga
121 ctacatggac tacgtccact cactcactca ctcatataca cattcacgta catagcagca
181 aaagttggtc caaagtttta aatatttctc gttgaattat aaagcaatat ttgacttcaa
241 atcaattagc aactctcgta ctcttgtaaa cataaaagtt ctacagttca cttttttatt
301 gaattaagaa ctctaatata tatggaatgt gagggtataa caagagcaat gttttttcgt
361 tgttcttatt aggggggggg ggttgtgagt gtgtgagacc tcattccata gagaggaaaa
421 aatggttatc ccgaaaatca ttttctcctc cacaagatct aaacttcttg tttaacatat
481 taacatgtat gcagtggaat aatatgttga aaatagaaga gactaaaata attcaattgg
541 aaagacgatt tgaaacttat tctatgtttt tacaaggtta aacttactgg aaacatttcc
601 gttttgaggt gcggtatcat gaacgggaaa catgtttagg gaaaaagatc tattaatttt
661 ctgaattttc tgaagatgac ccttcaccta aataacaact tacttttctt taaatacact
721 ttcacatctt tttgaattat atggggcctc ctttttttcg ttctatcaaa atgtgttata
781 tacgcaaaat aaagtttggt cagagtacag gattaggagg cagattagga gttaaggcaa
841 aaacagggtt ggatatatat tattatgtgg atgtacgcac atgtcgccat attaactctt
901 gccgctaact tgtccccttt gggtatttcc tgctgcgtat tctggtccaa aattagtcac
961 tctttgcgca tttccttcat ttgcatacga tccacccctt ttaggagagg ggcgtggcga
1021 ctgctgcttc cgtgccgtgt gcaaacatcc ccctgacccc ttcagcgcac gtttatgtgc
1081 attcgaagcc aggcagctca cttcagtaag tcattctgtg aattcactct tactgttgaa
1141 cattgtgcag ggttatacag ccagatactt gcctgaaagg aaaacaacag agaactttac
1201 ttagccatga agttgttggc aattttactc gtcctgccgg cgttatgctt tggccagcga
1261 cacgagggac caggaatggg tccaggaatg ggaccaggca tgggtccagg aatgggacca
1321 ggcatgggac caggtatggg accaggaatg ggaccaggga tgggcccagg ccagggccag
1381 ggccaggggc aaggacaggg acaagtcgga ggctcaaaat gcaagggagg ctggttcctc
1441 atcgggcagc aatgctttaa gatgatgtcc agagcgctga aatggaacga cgctgagttg
1501 atgtgtgagc agaacgcacc atgcggaacc cctgttctcg gaggcgtcat gacaatcccc
1561 gacatccaga catcgaacgc cgtcatcaac cacctcaaga gcctgtcatc caccgcaatg
1621 gccatcgata tcccattctg gaccggtctg cacaacaaat ggaacgcact tcttgagagg
1681 tacgaagggt ggaagtggcc ggccggatgg agcaccacac aacaacctct acggttcgtc
1741 aactgggccc caagggaacc caacaaccag cttctggatc agcagcactc gtactgcgca
1801 cgcatgaaca ggatgggaca gtggtacgtc gtaagatgtg acgagcctat gtacttcgcg
1861 tgctctatgc cggtcagccc accactcgtt ggaggagcta acaccaaccc cggtatggga
1921 atgcttgtgg agaaccccgc cccgatcatc aacggataca ccgaattcga aagtggtctg
1981 ctgatgagga acggcgtagg aggcccgtaa gactctcaca aaactttaat acagtgtcat
2041 gactagctcg tgatctaaaa attcaaaatg gcggacgtgt gaactttgac ccaaagccgt
2101 tggaaagaga gagcgagaga aaaccatggc aaaccttttt gaaaggattg tccaccaaac
2161 cagcaatatt cgcaattacc acgatctttc gcttcaagat attttaattc attgatattt
2221 aattttaaat gaaactgata gtctaaacta tttcgtagtc agggatgggt tttcataaag
2281 aatttcaaaa aattgcagtg atcgtaagtt gaaatagttt tgtgatgttt atattttcta
2341 tgttaaattt gaaacaagag gatgtacaat atactatttt gcaacaaatg taaaatttcc
2401 attaaacaaa ttaatttcat tgtacgcaat tgtgtgaatt tatccttttt tgattgctac
2461 tggtgagctg agttgttttc ttggattaat ggctgcttga ttgattgatt tggtggacag
2521 aaatattgaa tgattgattg atttgcagat ggactaattg gttgattgat ttgatgattg
2581 atactttgat tgattgtttg atcggttgat tgattggttg gttagttaat cgattggatg
2641 gttgatgaag tactgaatca ataaatgaat gaacgattga ttgattgatt gaaagattgg
2701 ttggttggtt atttaattga ttggatgatt cacggaatag agattcaata aattaatgag
2761 gatttgattg attgaaagat tggttggttg gttagtttat tgattgatga ttcacagaat
2821 agagattcaa taaattaatg agcgattt
//