LOCUS SUSMTA 5150 bp DNA INV 17-JUN-1993
DEFINITION Strongylocentrotus purpuratus (clone lambda-MT206)
metallothionein-A (MTA) gene, complete cds.
ACCESSION M30606
NID g161545
KEYWORDS metal-binding protein; metallothionein; metallothionein-A.
SOURCE Strongylocentrotus purpuratus DNA.
ORGANISM Strongylocentrotus purpuratus
Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
Strongylocentrotidae; Strongylocentrotus.
REFERENCE 1 (bases 1 to 5150)
AUTHORS Harlow,P., Watkins,E., Thornton,R.D. and Nemer,M.
TITLE Structure of an ectodermally expressed sea urchin metallothionein
gene and characterization of its metal-responsive region
JOURNAL Mol. Cell. Biol. 9, 5445-5455 (1989)
MEDLINE 90066495
REFERENCE 2 (bases 1 to 5150)
AUTHORS Bai,G., Stuebing,E.W., Parker,H.R., Harlow,P. and Nemer,M.
TITLE Combinatorial regulation by promoter and intron 1 regions of the
metallothionein SpMTA gene in the sea urchin embryo
JOURNAL Mol. Biol. Cell 13, 993-1001 (1993)
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by M.Nemer, 11-DEC-1989.
FEATURES Location/Qualifiers
source 1..5150
/organism="Strongylocentrotus purpuratus"
/db_xref="taxon:7668"
/cell_type="sperm"
misc_signal 492..499
/gene="MTA"
/standard_name="metal-responsive element (MRE)"
misc_signal 529..536
/gene="MTA"
/standard_name="metal-responsive element (MRE)"
misc_signal 1466..1473
/gene="MTA"
/standard_name="metal-responsive element (MRE)"
misc_signal 1566..1573
/gene="MTA"
/standard_name="metal-responsive element (MRE)"
TATA_signal 1592..1596
/gene="MTA"
mRNA join(1619..1727,2848..2934,4020..4128,4679..5094)
/gene="MTA"
gene join(1619..1727,2848..2934,4020..4128,4679..5094)
/gene="MTA"
exon 1619..1727
/gene="MTA"
/number=1
CDS join(1697..1727,2848..2934,4020..4096)
/gene="MTA"
/codon_start=1
/product="metallothionein-A"
/db_xref="PID:g161546"
/translation="MPDVKCVCCKEGKECACFGQDCCKTGECCKDGTCCGICTNAACK
CANGCKCGSGCSCTEGNCAC"
intron 1728..2847
/gene="MTA"
/number=1
exon 2848..2934
/gene="MTA"
/number=2
intron 2935..4019
/gene="MTA"
/number=2
exon 4020..4128
/gene="MTA"
/number=3
intron 4129..4678
/gene="MTA"
/number=3
exon 4679..5094
/gene="MTA"
/number=4
BASE COUNT 1495 a 935 c 1024 g 1662 t 34 others
ORIGIN
1 gatcctaagt cgactgaggt tcacggatca ggttgataaa cttgcagctc agccatggag
61 gtatgcatca atccttgtat gataaaaaat gtactcgatg accccgtgaa gaacgaaggc
121 ggcgggcaaa aatatacagg cattttaaga agtccagagc tacatgaaca tcttggttat
181 aaatatatcc tgctgtggac tgcgaattga atgatgttta taatcctgtt tttacctttg
241 gaaattgcag taataataga atgtaatacc tccaacattt ggtaaagagt ttggagttgg
301 tatttcgtat gattattcag cgaattttag aaaacaagag cagttcaaaa atatttatcc
361 atctagaagc cacacaagac cggtcacatg attcaaccca gccaaactca gaaaagctgc
421 ccgcacataa gtgtactgta cgtgtcacgt atttaaataa tcttgaatat tcaaactatt
481 gttatattat tcgtgtgcaa taatgaggac gagcatgcaa gcgctactcg agtgcaaacc
541 caaccacctc tagtctctac ttaaggtgtt taaatcttta tctcaaagta tgacagaaaa
601 gacgtcataa tcatatttgt atcacctccc ccctctcccc ttgggtaggg aaggcgaaat
661 ttgagaaaac aatagaaatt gaacattaat aatgtattaa taatgtttat gctccatttc
721 atgcatttcg tgtgaaacaa cgagttaaaa atatgcatgc agtaaatcct ttcgtctcca
781 ctctgggtca atcgtcaggg gcagatccag gattcatttg aaatgaaatc gaggagggag
841 gggggggggg gagatttcca ctgatgcctg gtgtgtagca ctatggcaat ttgactgggg
901 gtaagtgtct ctaatacgcc ctataggcaa atattgacag gtctacgtaa tttagtatgg
961 aaaaataacc attttatcat acaggttgat catcaatata tcattatgca gtgaacacca
1021 tgaatatttc gaaatggagg gatagttgca tttctggcgg agatatccgg gtcgggaatc
1081 atctactttt attccccctc cccatttttt tcttcttctc cttgttcttc ttctttttcg
1141 tttcttaatc atggtattaa tcatttgcaa atcaattaca tgcctttata tatatataat
1201 aatcatggtg ctacatgatt ttgaaatact ttatggaaac cttacgtttt accagacctt
1261 gacacaaagt tgacagtgtt acctttatca ttcacttccg tactgtttca gcacatgagc
1321 aacactttgt tctttgaggg caaacgtttt gggggagggg agggcatccc acggcgcttt
1381 aaaaacaaaa aggatttttg cttcccttcc atatcccgta taatttatcc gacacgagcc
1441 tagtcatcac cattcctgat agaagcgtgt gcaaaactag aaaggcccac ctagatcgag
1501 ctcctcaggc accgctaaca ggttgcctag ctcgtgccta aacgtggtgt gtttacctat
1561 ttgtttgcac acggatattg taatgaaaac atataaaacc ccgaatttca ccagtttttg
1621 tcacaagctg aagcgtttga gtgtgattta cattaccttc tgaagaaaca tacaactcga
1681 gttcaatttc atcaccatgc ctgatgtcaa gtgtgtctgc tgcaagggta agcaaaaaat
1741 cggaatcaat gttctcatta aaataccgag ttacagaata ccgtgtacgt acggtgtagc
1801 gtttctagaa acaaaccgct cgatgttacg tgcatacgat gatacgatac agccatggcc
1861 atgaccatga gaggcccccg atcacgacac acgcagctag ctagaccgaa ttttccgtct
1921 ctttcgttcg ttaagcctct tatttgggta gggtcggctc tctctttaga gacggaagcc
1981 gactagacct agggcgcgtt catgcttgat tttgaaggca gaatgagcga tttgtaacgc
2041 tcatcgtaaa cgctcatcgt aatcgcagtt gcacggtgca ctgttcatgc tcacttctcc
2101 aggcaattct ccttgggtgc cttcagctgg attcgcaaac ctgtgtgcgc atgctacgag
2161 agcccataga ggtcagtgcg ttgaccccat ttaaacgcgt ttacaaattg taaacgcatc
2221 tttttgtgag tttacgatcg gcgatcatga atcggctttt tcggtgattc tacaaaatca
2281 agcatgaacg cgcccctata ctcattccct tgctaacacc aaggtgcatt tatttttann
2341 nntatnccna naannncccc aaatgtcatt ttacctcgtc gcatgttata ttgtaaatta
2401 ggcctaggct ataataaatc ggttccactt accaacttga ctgaaataat ttaatggtcc
2461 ctttttagga aattatgggt ctgaaatcga aatccatgtt gaaaatcttg caaaacgtat
2521 tttaactctc gctgcgcacg acgtaatcta aatctcatcc cttctagtct acaccgaagg
2581 caaaatgatt atgaaggaaa aactgtgcgc gagcttcacg ttcgttatca cagtttttct
2641 tcataatcat tttcatgttt tcttgcccgt ttcttcatac ttgttacatc aacagttatg
2701 tcaataacta agtttttacc aacaagcact gctggatttg tggtttcaaa ttgcaagatt
2761 gttattactt ttttaaaatt ttatatcatt atatagacta tatatatata tatatatata
2821 tatttttttt tttaattctg aacacagagg gcaaggagtg tgcctgcttt ggacaagatt
2881 gctgtaaaac tggtgaatgc tgcaaggatg gaacctgctg tggaatatgc acaagtaagc
2941 ctttcatcat tttcagtgaa ccctttaaaa ttaaagcagg cgcgtacgag gattttttaa
3001 gggggggggg ggggggttta acatttataa atcgggccga aaattctgca acgactcagc
3061 cgaccgctga ataagtgnng gggggggggg gggtagtaag aggacacttt ttattttttt
3121 ttggtctcga aattagaaaa tttgacattt tgctccgttg gggggggggg ggggaaggtc
3181 agctttttta ggtcagccat gggaattttt ttttttaata aaaaaaaaaa acatccagan
3241 nnnggggggg ggtttttaag gaaatttagg gggggggttt acacacaaaa aattaccaaa
3301 gggtgggggt ttaaccnnnn nnnncccctg tgtaagcncc tgcatcccag taattaaggc
3361 ttttttatac taaactgttt acacaaggct tttaaaagta tttatattca tagaaaaaaa
3421 ttgtcccaat tgctcatgca tttttttaat ttctttttac tctcaccttt aaatgggtgc
3481 aggcttaaat gtcaggctaa ctggcaatga ttatttgtgc tcattatcaa tattctagtg
3541 tatccatggt tttgttatgg tcgggggggg gggatctgac aaaaaatatt gacagccaag
3601 aatataggtc tatatgattt gtcagggaga tatgaatttc acatctccta ggttttattg
3661 ttgttgtcac caattaaatt attttaaaaa tcactgacaa gcttggaaaa ggattttgaa
3721 aataatttaa aacatgttca tctcattttc ctggtgaatt ttgggtctga aaagcgctat
3781 ataagaacta gctattatta tatatatata tatatatata taattagatt ttttattttt
3841 ttggaggggg gggggggggn ctttttccat gtatgcctaa atgaggggcg cagaccaatt
3901 gtgcccttta tatatcctgc tgtgtagtct gcatgaaaga gaaaatgcag ttataccaac
3961 aatagaatag agacatttca atttgatgtt attacttggt taattttatt tgtccacaga
4021 cgctgcgtgc aagtgtgcta atggctgcaa gtgtggaagc ggctgctcat gcactgaggg
4081 gaactgtgca tgctagacga gcttggccct ttgctatagg gatggcaggt gagattttat
4141 ttattttcat ggctacatag taaaaattgt ttccaggcaa aaacgttagc tagttatggg
4201 atatgacagg gtctgaaatg taagactgta gatttgcact gcataaagct gtgccatcat
4261 ttttttttaa atagaatttt ccgagagagc atcaaaatta atgtccaaat gtcgaaagcc
4321 aactgaatat gttaaatatt ggctgagatg tgaccatctg aataaatagg ttccctatag
4381 gaactcagtg ttattttgct gtggttcata acatcacgna ccagagcaaa ataacactga
4441 gttcctacac aaaatctatt tattacatta ctgcattgcg atatacaaaa aaggtaaaat
4501 tagaaaatct ttgcaagtta tggcaatatg tatagacctt ccgtgtcttt catgactttt
4561 gtatattttt ttcaacaacc ggaacaaatt cattaaccat ttctgtgttt tcatactttt
4621 ttgtaaaaat attttcaagc attttgtttt tctctccgtg tctgtttctc ttctacagat
4681 tgagcataca gctgatgaag tcactcagcg catctgccaa catgctccaa gcagacctga
4741 ctttgtatat actttcgacc cgatgtatta tattttttgt tgtaagatag ttgtatatta
4801 agttaattta atctttattt ttttctgaaa atatgagact aattggcatn nnntaatagg
4861 actacttaaa caacaattat ttattaaggc tgacagtaat gcctgataga atttcacttc
4921 attttttngc tgccctgttt tgtaaattct tcagtctgtt tctaacttct atncaagttg
4981 tcaaatttgg aatgaaaata ataataataa tacatgtgga gagtacttgt agtgcgcaaa
5041 ctccacatga ctaatgtgtt aaagactcgc tcatgattaa atttaaanta atgctacaaa
5101 gtatattcag tatgcgttgc ttagtttttt ctcatgattt atttggcatg
//