LOCUS       SPSPE2C1      532 bp    DNA             INV       19-JUN-1992
DEFINITION  Strongylocentrotus purpuratus Spec2c gene 5'-flank and exon 1.
ACCESSION   X12772
NID         g10295
KEYWORDS    calcium binding protein; Spec2 gene.
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Hardin,P.E., Angerer,L.M., Hardin,S.H., Angerer,R.C. and Klein,W.H.
  TITLE     Spec2 genes of Strongylocentrotus purpuratus. Structure and
            differential expression in embryonic aboral ectoderm cells
  JOURNAL   J. Mol. Biol. 202 (3), 417-431 (1988)
  MEDLINE   89011969
COMMENT     see x12772, x14534 - x14538 for Spec2C gene exon seq. Data kindly
            reviewed (09-MAY-1989) by Klein W.H.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
                     /clone_lib="lambda EMBL3"
     promoter        386..391
                     /note="TATA-box"
     precursor_RNA   416..>532
                     /note="primary transcript"
     mRNA            416..526
                     /note="Exon 1"
     CDS             524..526
                     /note="Spec2c protein (AA 1 - 1)"
                     /codon_start=1
                     /db_xref="PID:g578508"
                     /db_xref="SWISS-PROT:P04111"
                     /translation="M"
     intron          527..>532
                     /note="Intron I (approx. 6.5 kb)"
BASE COUNT      183 a    116 c     82 g    151 t
ORIGIN
        1 ttatctatta gtgacagtgg tactatcctc ggtataaatt gcttcattgt ataaattgta
       61 taaattgctt cattataacg aagagaggtt ttaactaacg tcctaacgta atgaactgat
      121 cgcaactaat attcaaccac ttcatccttt ctttcctaat gaattcttta aaataacaag
      181 ccgaataaca atacagaaaa taactactaa caatttactg acattgttaa ttgcacaaca
      241 caacgctatt ataggctttc gtcgatccaa cagtaaaagt attaagagag gaccagtaac
      301 tacgccctct cacattgcct ggcggaaatc atcgaatgat caatcgaatt gacgatggaa
      361 acgcacttga ccttgtggaa acgccattaa acccaacaac aacaactcca cccttgccac
      421 tccttgaaag caagtcgact agaagagagg tataggtacc tgagctccag agaaatttta
      481 ctttctatct tcaattttgc cggaccaatc ccaatttcta aagatggtaa gt
//