APPENDIX I

List of Reagents, in alphabetical order

(* reagents used in Mol. Bio. Lab)


*
Reagent - Storage Condition

*2X YT - store at room temp.

*agar plates - store at room temp.

*ammonium acetate, 7.5 M - store at room temp.

*ampicillin (Fisher #BP1760-25) - store at -20° C

*Blotto- store at 4° C

*CaCl2 solution - store at +4° C

*chloroform, 100% - store at RT°

*competent bacteria (capable of taking up DNA) - store at -70° C

*denaturing solution (for DNA) - store at RT°

*DNA loading dye - store at +4° C

*DNAse, 10 mg/ ml - store at -20° C

*ECL Primary Wash- store at RT°

*ECL Secondary Wash- store at RT°

*EDTA, 0.5 M pH 8.0 - store at RT°

*ethidium bromide - store at +4° C
WARNING THIS IS A KNOWN MUTAGEN WEAR GLOVES

*EtOH (ethanol) - store at RT° or +4° C

*EtOH 70%, - store at RT° or +4° C

*gelatin, 2% - store at RT°

*glacial acetic acid - store at RT°

*glucose, 10% - store at RT°

*glycerol, sterile RT°

*IPTG (isopropylthio- b - D -galactoside) - store at -20° C

*LB - store at RT°

*magnesium sulfate, 1 M - store at RT°

*maltose, 20% (w/v) - store at RT°

*miniprep solution I - store at RT°

*miniprep solution II - store at RT°

*miniprep solution III - store at +4° C

*neutralizing solution (for DNA) - store at RT°

*Ponsceau S- store at RT°

*phenol/chloroform/isoamyl alcohol (Ameresco) - store at +4° C

*phenol, saturated and buffered (pH 8.0) (Ameresco) - store at +4° C

*potassium acetate, 5 M stock - store at RT°

*proteinase K, 10 mg/ ml - store at -20° C

*RNAse, 10 mg/ ml (SIGMA# R-5125) - store at -20° C

*sarkosyl (Sigma # L-5125) - store at RT°

*SDS (Sigma # L-5750) sodium dodecyl sulfate, also called lauryl sulfate - store RT°

*SM - store at RT°

*sodium acetate, 3M - store at RT°

*sodium chloride, 5 M - store at RT°

*sodium hydroxide (CAUTION - VERY CAUSTIC) - store at RT°

*SSC, 20X stock - store at RT°

*TBE - Tris, Boric acid, EDTA - store at RT°

*TE - Tris, pH 8.0, EDTA - store at RT°

*TEN - store at +4° C

*Tetracycline - store at -20° C

*top agarose or agar - store at RT°

*10X Transfer buffer stock (western blots)- store at RT°

*1X Transfer buffer (western blots)- store at 4° C

*Tris, 1 M - store at RT°

*10X Tris Saline- store at RT°

*Turbo Denaturing Buffer- store at RT°

*Turbo Transfer Buffer- store at RT°

*5X Turbo Neutralizing Buffer- store at RT°

*X-gal (5-bromo-4-chloro-3-indoyl- b - D -galactoside) - store at -20° C


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