Methodology for Functional Genomics
Step 1. Create a microarray of every gene in yeast
genome.
![](microarray.gif)
Step 2. Isolate mRNA from yeast grown under two different
conditions; generate labeled cDNA of two different colors.
![](method.gif)
Step 3. Mix the two probes and incubate with microarray.
![](hybridize.gif)
Step 4. Wash and detect fluorescence.
Determine which genes are transcribed in each condition....
![](green.gif)
or both conditions...
![](yellow.gif)