Protocols for GCAT
Growing Yeast

Media Recipes

Media Preparation

Strains

Growth Conditions


Media Recipes:

Use one of the following media for growing wild type yeast strains. Note that pre-made media can be purchased from many companies, such as Bio101.

 

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Media Preparation

  1. Liquid
  2. Solid

 

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Strains:

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Growth Conditions

Starting a yeast liquid culture from a frozen stock:(from Karen Bernd <kabernd@davidson.edu>)
The following are protocols provided to undergraduate researchers using yeast for the first time. They provide every detail I could think of. (K.B.)

Notes:

  1. All materials used must be sterile (Including inoculating "toothpicks")
  2. YEPD is yeast "rich media". The media used will vary if yeast strains need to remain under selection.
  3. Aeration is important so cultures should be placed in tubes or flasks that provide extra space and should be agitated during incubation ("roller barrel" is best but racks or tubes in a shaking incubator [175rpm or better depending on tube/flask used])
  4. Optimal growing temperature for wild type yeast is 30° C. They can be grown at room temp if necessary but they will not be happy at
    37° C)

Notes:

  1. wooden applicator stick (long toothpick) or sterile pipet tip can also be used
  2. frozen cultures can be reused-replace the cap and place back in the -80°C for 3-5 years)

 

(Trying to separate single colonies: changing toothpicks and turning the plate means that you pick up and spread out fewer cells with each pass)

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© Copyright 2001 Department of Biology, Davidson College, Davidson, NC 28036
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