Bacteriophage T7 Gene, promoter and RNAse List
Far Left Side of Genome
Class I genes - transition to phage metabolism
- E. coli promoter A0 (transcribes leftward)
- T7 promoter phiOL
- E. coli promoter A1
- E. coli promoter A2
- E. coli promoter A3
- RNase III site R0.3
- gene 0.3, inhibits EcoB and EcoK host restriction
- gene 0.4
- gene 0.5
- E. coli B promoter
- possible gene 0.6B
- gene 0.6A
- gene 0.7, protein kinase
- E. coliC promoter
- RNase III site R1
- gene 1, T7 RNA polymerase
Class II Genes - T7 replication
- T7 promoter phi1.1A
- RNase III site R1.1
- T7 promoter phi1.1B
- gene 1.1
- gene 1.2, inhibits host dGTP triphosphohydrolase/target for F plasmid exclusion
- T7 promoter phi1.3
- RNase III site R1.3
- E. coli transcription terminator TE
- gene 1.4
- T7 promoter phi1.5
- gene 1.5
- T7 promoter phi1.6
- gene 1.6
- gene 1.7
- gene 1.8
- gene 2, inhibits E. coli RNA polymerase
- T7 promoter phi2.5
- gene 2.5, single-stranded DNA-binding protein
- gene 2.8
- gene 3, endonuclease
- gene 3.5, lysozyme/inhibits T7 RNA polymerase [7]
- T7 promoter phi3.8
- possible RNase III site R3.8
- gene 3.8
- gene 4A, primase/helicase [14,15]
- gene 4.1
- gene 4B/helicase
- T7 promoter phi4c
- gene 4.2
- T7 promoter phi4.3
- gene 4.3
- gene 4.5
- RNase III site R4.7
- T7 promoter phi4.7
- gene 4.7
- gene 5, DNA polymerase
- gene 5.3
- gene 5.5, permits growth on lambda lysogens /binds host nucleoid protein H-NS
- possible gene 5.5-5.7
- gene 5.7
- gene 5.9, inhibits host recBCD nuclease [13]
- gene 6, exonuclease
- gene 6.3
Class III genes - phage particle proteins, packaging and maturation of phage
- T7 promoter phi6.5
- RNase III site R6.5
- gene 6.5
- gene 6.7
- gene 7, host range
- gene 7.3, host range
- gene 7.7
- gene 8, head-tail connector protein
- T7 promoter phi9
- gene 9, scaffolding protein
- T7 promoter phi10
- gene 10B, minor capsid protein [12]
- gene 10A, major capsid protein
- T7 transcription terminator Tphi
- gene 11, tail protein
- gene 12, tail protein
- possible RNase III site R13
- gene 13, internal virion protein
- gene 14, internal virion protein
- gene 15, internal virion protein
- gene 16, internal virion protein
- T7 promoter phi17
- gene 17, tail fiber protein
- gene 17.5, lysis protein
- gene 18, DNA maturation protein
- E. coli promoter E
- RNase III site R18.5
- gene 18.5, homologous to lambda Rz lysis protein
- gene 18.7
- gene 19, DNA maturation protein
- gene 19.2
- gene 19.3
- T7 promoter phiOR
- gene 19.5
Far Right Side of Genome