The Next Steps:
- Perfect digestions and ligations of PCR product
- Combine the intermediate plasmids to form the final constructs
- Use constructs to transform mutant Chlamy cells
- Observe possible complementation of the mutation and mating of Chlamy cells
Acknowledgements:
I wish to thank Dr. Campbell for his guidance, support, and unending patience throughout the summer. I also thank Dr. Ginger Armbrust and Michelle Long for their collaboration and Allison Coble for previous work on this project. Most of all, I thank the Howard Hughes Medical Institute for the opportunity to do this research; without HHMI this would not have been possible.
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