Immunofluorescence Labeling of Calcium Pump

Figure 2. The primary anti-calcium pump antibody binds specifically to chicken calcium pumps. Chicken embryos were fixed in 4% formaldehyde, treated with lysine, mounted on subbed slides, incubated overnight with a primary antibody diluted in a BSA/saponin solution. The embryos were then washed with PBS three times, incubated overnight with a fluorescene conjugated secondary antibody diluted in a BSA/saponin solution, washed three times with PBS, covered with mounting medium, sealed with a coverslip, and photographed with an epifluorescence microscope.

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