Provide thorough, but concise, answers to 7 of the following 8 questions.
All questions are worth 10 points.
There is no time limit to this test.
The review is closed book.
Calculators are permitted.
All answers must be typed.
Please staple all pages together and do not put your name on any page other than this cover page.
Return completed review to Mrs. Hartsell (Watson 162) no later than 4:00PM, Friday October 26, 2001.

Name (please print):

Pledge and signature:

1. Briefly define the following terms:

Locus
Chromosome
Allele
Gene

Draw a picture to fully demonstrate you understand these terms.

2. What is a restriction endonuclease (AKA restriction enzyme)? Explain how these enzymes relate to RFPLs.

3. Describe the two major mechanisms by which of meiosis leads to genetic variation?

4. For the following cross, AaBb x Aabb, show a Punnet square and provide the expected genotypic and phenotypic ratios of the progeny. Assume the genes are located on different chromosomes.

5. For the following cross, AaBb x aabb, the following numbers of offspring of the various phenotypes are observed:

Explain these results.

6. Some members of the Davidson College community are found to be wildcatase deficient. A sequence analysis of the wildcatase gene in numerous individuals reveals that many different mutations in this gene result in wildcatase deficiency. Describe two different types of mutations that could result in this disease and the effect on transcription, translation, and/or enzymatic activity that you would expect each mutation to have.

7. Pretend you used PCR to amplify the D1S80 locus of your DNA, your biological mother's DNA, and your biological father's DNA. The resulting PCR products then were analyzed by gel electrophoresis. Draw a picture representing the resulting gel. Briefly explain the important parts of your picture.

8. A) To test the effects of cycloheximide, an inhibitor of translation, on chlamy flagellar regeneration, you must make 100mL of a 150mM cycloheximide solution. How would you make this solution?

B) Using this 150mM cycloheximide stock solution, how would you make 1mL of 5mM cycloheximide?

NOTE: The formula weight of cycloheximide is 281.4.