Bio113 Lab Schedule - Dr. Campbell at Davidson College

Your oral presentation will be divided into four sections: Introduction; Materials and Methods; Results; Discussion. Each person will present one section. The oral presentation must be pledged to indicate that each person made substantial contributions. You will not know which section you are presenting until 1 hour before lab, therefore you should learn all 4 parts.

For the final written lab report, each person must write his or her own (including figure legends), though you may confer about the content. The figures may be shared resources since they were produced as a team. Put your name as the first author and your labmates' names as coauthors.

Date	Topics		Lab Manual Word Files
Sept 1	Lab orientation & begin Excel graphing	1) Safety Issues 2) Submit info to CATME for Team maker 3) need 1 volunteer per group to come to lab Wednesday 4:30 to assemble promoter 4) GenBio-MAPS pre-survey if this is your first college biology course.	all needed lab files for week #1 practice calculations - optional
Sept 8	Day before lab: assemble oligos into promoter (1 person/lab group) Day of lab: perform GGA, transform cells, plate cells	Before Lab... 1) answer 4 Questions 2) one person from each lab group comes to Wall 104 4:30 Wednesday to start assembling a promoter During Lab... 3) Start GGA 4) hear GGA presentation 5) paper GGA activity 6) generate GGA PPT file 7) transform and plate cells	all needed lab files for week #2
Sept 15	Day before lab: start cells growing overnight Day of lab: analyze cell cultures & generate graphs	Before Lab... 1) answer 4 Questions 2) one person from each lab group comes to Wall 104 4:30 Wednesday to start cells growing overnight During Lab... 3) PCR to confirm promoter cloning 4) freeze each clone 5) measure promoter phenotype & graph	all needed lab files for week #3
Sept 22	Complete plasmid genotyping, work on oral presentations	Before Lab... 1) Answer 4 Questions 2) Make sure you understand GGA Overview and Detailed explanations During Lab... 3) Run gel electrophoresis to determine plasmid genotype 4) Hear in depth presention about GGA 5) Start PPT file for oral presentation (2 weeks from today) 6) CATME peer review	all needed lab files for week #4
Sept 29	Finalize PPT presentation, practice if you have time	Before Lab... 1) Answer 4 Questions 2) Make sure you fully understand GGA During Lab... 3) Finalize PPT presentation for next week 4) Practivce presentation so everyone knows all parts 5) CATME peer review	all needed lab files for week #5
Oct 6	Give oral presentations Oral Report Grading Rubric	Before Lab... 1) Answer 4 Questions 2) prepare oral presentation, asignments emailed 1 hour before During Lab... 3) give oral presentation, read peer comments (iBOP Bingo) 4) submit your research proposal for promoter v2 using this Research Proposal Template (graded) 5) CATME peer review	all needed lab files for week #6
Oct 13	Day before lab: assemble oligos into promoter (1 person/lab group) Day of lab: perform GGA, transform cells, plate cells	Before Lab... 1) answer 4 Questions 2) one person from each lab group comes to Wall 104 3:30 Wednesday to start assembling a promoter During Lab... 3) Start GGA 4) refine GGA PPT file 5) transform and plate cells 6) CATME peer review	all needed lab files for week #7
Oct 20	Day before lab: start cells growing overnight Day of lab: miniprep plasmids; analyze cell cultures & generate graphs	Before Lab... 1) answer 4 Questions 2) one person from each lab group comes to Wall 104 4:30 Wednesday to start cells growing overnight During Lab... 3) measure promoter phenotype & graph 4) miniprep plasmid DNA 5) CATME peer review	all needed lab files for week #8
Oct 27	Send DNA for sequencing	Before Lab... 1) Answer 4 Questions During Lab... 2) Nanodrop DNA 3) Mix sequencing primers with diluted DNA template 4) CATME peer review	all needed lab files for week #9
Nov 3	Analyze DNA Sequencing Results	Before Lab... 1) Answer 4 Questions During Lab... 2) Align expected DNA sequence with experimental results 3) Generate graphic for your final report 4) CATME peer review	all needed lab files for week #10
Nov 10	Prepare final lab preport (individually) Lab Report Template	Before Lab... 1) Answer 4 Questions During Lab... 2) Prepare individualized lab report 3) Lab report due end of next lab (17 Nov) 4) CATME peer review	all needed lab files for week #11
Nov 17	Submit Final Lab Report Lab Report Template	Before Lab... Work on your lab report During Lab... email individualized final lab report	all needed lab files for week #12
Nov 24	Thanksgiving Break	No Lab Meeting	No Lab Manual for the holiday, you're on your own!
Dec 1	Dr. C. attends ASCB Meeting	No Lab Meeting	Spend lab time wisely
Dec 8	Fellowships for you & Course Evaluations	1) Fellowships for you (free money) 2) Course Evaluations	Please submit evaluations to help improve this course

Bio 113 Lab Schedule - Dr. C. Fall 2022

List of Protocols Used in Bio113

Practice Calculations

Dr. Campbell's Bio113 Page