Bio 113 Lab Schedule - Dr. C. Fall 2022

Lab meets either Tuesday at 1:40 pm or Wednesday at 1:30 pm in Wall 104.

Lab Safety Guidelines and Agreement. 

Learning Objectives for Lab Course

List of Protocols Used in Bio113

We will use CATME to facilitate fair assessment of lab group projects.

schedule

Your  oral presentation will be divided into four sections: Introduction; Materials and Methods; Results; Discussion. Each person will present one section. The oral presentation must be pledged to indicate that each person made substantial contributions. You will not know which section you are presenting until 1 hour before lab, therefore you should learn all 4 parts.

For the final written lab report, each person must write his or her own (including figure legends), though you may confer about the content. The figures may be shared resources since they were produced as a team. Put your name as the first author and your labmates' names as coauthors.

Grading will be based on these grading rubrics.

Date
Topics
Lab Manual
Word Files
Sept 1

Lab orientation & begin Excel graphing

1) Safety Issues
2) Submit info to CATME for Team maker
 
3) need 1 volunteer per group to come to lab Wednesday 4:30 to assemble promoter
4) GenBio-MAPS pre-survey if this is your first college biology course.  

all needed lab files for week #1

practice calculations - optional

Sept 8

Day before lab: assemble oligos into promoter (1 person/lab group)

Day of lab: perform GGA,  transform cells, plate cells

Before Lab...
1) answer 4 Questions
2) one person from each lab group comes to Wall 104 4:30 Wednesday to start assembling a promoter

During Lab...
3) Start GGA
4) hear GGA presentation
5) paper GGA activity
6) generate GGA PPT file
7) transform and plate cells
8) CATME first week

all needed lab files for week #2

Sept 15

Day before lab: start cells growing overnight

Day of lab: analyze cell cultures & generate graphs

Before Lab...
1) answer 4 Questions
2) one person from each lab group comes to Wall 104 4:30 Wednesday to start cells growing overnight

During Lab...
3) PCR to confirm promoter cloning
4) freeze each clone
5) measure promoter phenotype & graph
6) CATME first week

all needed lab files for week #3

Sept 22

Complete plasmid genotyping, work on oral presentations 

Before Lab...
1) Answer 4 Questions
2) Make sure you understand GGA
Overview and Detailed explanations

During Lab...
3) Run gel electrophoresis to determine plasmid genotype
4) Hear in depth presention about GGA
5) Start PPT file for oral presentation (2 weeks from today)
6) CATME peer review

all needed lab files for week #4
Sept 29

Finalize PPT presentation, practice if you have time

Before Lab...
1) Answer 4 Questions
2) Make sure you fully understand GGA

During Lab...
3) Finalize PPT presentation for next week
4) Practivce presentation so everyone knows all parts
5) CATME peer review

all needed lab files for week #5
Oct 6

Give oral presentations

Oral Report Grading Rubric

Before Lab...
1) Answer 4 Questions
2) prepare oral presentation, asignments emailed 1 hour before

During Lab...
3) give oral presentation, read peer comments
(iBOP Bingo)
4) submit your research proposal for promoter v2 using this Research Proposal Template (graded)
5) CATME peer review

all needed lab files for week #6
Oct 13

Day before lab: assemble oligos into promoter (1 person/lab group)

Day of lab: perform GGA,  transform cells, plate cells

Before Lab...
1) answer 4 Questions
2) one person from each lab group comes to Wall 104 3:30 Wednesday to start assembling a promoter

During Lab...
3) Start GGA
4) refine GGA PPT file
5) transform and plate cells
6) CATME peer review

all needed lab files for week #7
Oct 20

Day before lab: start cells growing overnight

Day of lab: miniprep plasmids; analyze cell cultures & generate graphs

Before Lab...
1) answer 4 Questions
2) one person from each lab group comes to Wall 104 4:30 Wednesday to start cells growing overnight

During Lab...
3) measure promoter phenotype & graph
4) miniprep plasmid DNA
5) CATME peer review

all needed lab files for week #8
Oct 27

Send DNA for sequencing

Before Lab...
1) Answer 4 Questions

During Lab...
2) Nanodrop DNA
3) Mix sequencing primers with diluted DNA template
4) CATME peer review

all needed lab files for week #9
Nov 3 Analyze DNA Sequencing Results

Before Lab...
1) Answer 4 Questions

During Lab...
2) Align expected DNA sequence with experimental results
3) Generate graphic for your final report 4) CATME peer review

all needed lab files for week #10
Nov 10

Prepare final lab preport (individually)

Lab Report Template

Before Lab...
1) Answer 4 Questions

During Lab...
2) Prepare individualized lab report
3) Lab report due end of next lab (17 Nov)
4) CATME peer review

all needed lab files for week #11
Nov 17

Submit Final Lab Report

Lab Report Template

Before Lab...
Work on your lab report

During Lab...
email individualized final lab report

all needed lab files for week #12
Nov 24 
Thanksgiving Break

No Lab Meeting

No Lab Manual for the holiday, you're on your own!
Dec 1 Dr. C. attends ASCB Meeting

No Lab Meeting

Spend lab time wisely

Dec 8

Fellowships for you &
Course Evaluations

1) Fellowships for you (free money)
2) Course Evaluations

Please submit evaluations to help improve this course

Practice Calculations

Dr. Campbell's Bio113 Page


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Send comments, questions, and suggestions to: macampbell@davidson.edu