Bio113 Lab Schedule - Dr. Campbell at Davidson College

Your oral presentation will be divided into four sections: Introduction; Materials and Methods; Results; Discussion. Each person will present one section. The oral presentation must be pledged to indicate that each person made substantial contributions. You will not know which section you are presenting until 1 hour before lab, therefore you should learn all 4 parts.

For the final written lab report, each person must write his or her own (including figure legends), though you may confer about the content. The figures may be shared resources since they were produced as a team. Put your name as the first author and your labmates' names as coauthors.

Date	Topics		Lab Manual Word Files
Jan 16 - 17	Lab orientation & pipetting	1) Safety Issues 2) Submit info to CATME for Team maker 3) practice pipetting	all needed lab files for week #1
Jan 23 - 24	Design first experiment Graph in Excel (not Google)	Before Lab... 1) answer 4 Questions During Lab... 2) use Synergy plate reader and Excel	all needed lab files for week #2
Jan 30 - 31	Day before lab: assemble oligos into promoter (1 person/lab group) Day of lab: perform GGA, transform cells, plate cells	Before Lab... 1) answer 4 Questions 2) one person from each lab group comes to Wall 104, 4:30 Monday, to start assembling a promoter During Lab... 3) Start GGA 4) GGA presentation 5) paper GGA activity 6) generate GGA PPT file 7) transform and plate cells	all needed lab files for week #3
Feb 6 - 7	Day before lab: start cells growing overnight Day of lab: analyze cell cultures & generate graphs	Before Lab... 1) answer 4 Questions 2) one person from each lab group comes to Wall 104, 4:30 Monday, to start cells growing overnight During Lab... 3) PCR to confirm promoter cloning 4) freeze each clone 5) measure promoter phenotype & graph	all needed lab files for week #4
Feb 13 - 14	Complete plasmid genotyping, work on oral presentations	Before Lab... 1) Answer 4 Questions 2) Make sure you understand GGA Overview and Detailed explanations During Lab... 3) Run gel electrophoresis for plasmid genotyping 4) Hear detailed presention about GGA 5) Make PPT for oral presentation (due in 2 weeks)	all needed lab files for week #5
Feb 20 - 21	Prepare oral presentations Oral Report Grading Rubric	Before Lab... 1) organize all your lab data During Lab... 2) prepare oral presentation (iBOP Bingo) 3) draft research proposal for promoter v2 using this Research Proposal Template (graded)	all needed lab files for week #6
Feb 27 - 28	Give oral presentations Oral Report Grading Rubric	Before Lab... 1) practice oral presentation; asignments emailed 1 hour before During Lab... 2) give oral presentation, read peer comments (iBOP Bingo) 3) submit your research proposal for promoter v2 using this Research Proposal Template (graded)	all needed lab files for week #7
Mar 5 - 6	Spring Break	No Lab Meeting	No Lab Manual for the holiday, you're on your own!
Mar 12 - 13	Day before lab: assemble oligos into promoter v2 (1 person/lab group) Day of lab: perform GGA, transform cells, plate cells	Before Lab... 1) answer 4 Questions 2) 1 person from each lab group comes to Wall 104, 4:30 Monday, to start assembling v2 promoter During Lab... 3) Start GGA 4) refine GGA PPT file 5) transform and plate cells	all needed lab files for week #8
Mar 19 - 20	Day before lab: start cells growing overnight Day of lab: miniprep plasmids; analyze cell cultures & generate graphs	Before Lab... 1) answer 4 Questions 2) 1 person from each lab group comes to Wall 104, 4:30 Monday, to start cells growing overnight During Lab... 3) measure v2 promoter phenotype & graph 4) miniprep plasmid DNA	all needed lab files for week #9
Mar 26 - 27	Send DNA for sequencing	Before Lab... 1) Answer 4 Questions During Lab... 2) Nanodrop DNA 3) Mix sequencing primers with diluted DNA template	all needed lab files for week #10
Apr 2 - 3	Analyze DNA Sequencing Results	Before Lab... 1) Answer 4 Questions During Lab... 2) Align expected DNA sequences with experimental results 3) Generate graphics for your final report	all needed lab files for week #11
Apr 9 - 10	Prepare final lab preport (individually) Lab Report Template	No Lab Meeting	Start working on final lab report
Apr 16 - 17	Submit Final Lab Report Lab Report Template	Before Lab... 1) Work on your lab report During Lab... 2) finish lab report 3) email individualized final lab report	Spend lab time wisely
Apr 23 - 24	Fellowships for you & Course Evaluations	1) Fellowships for you (free money) 2) Course Evaluations	Please submit final course evaluations

Bio 113 Lab Schedule - Dr. C. Spring 2024

List of Protocols Used in Bio113

Dr. Campbell's Bio113 Page