Bio 113 Lab Schedule - Dr. C. Spring 2024

Lab meets either Tuesday at 1:40 pm or Wednesday at 1:30 pm in Wall 104.

Lab Safety Guidelines and Agreement.

Learning Objectives for Lab Course

List of Protocols Used in Bio113

schedule

Your  oral presentation will be divided into four sections: Introduction; Materials and Methods; Results; Discussion. Each person will present one section. The oral presentation must be pledged to indicate that each person made substantial contributions. You will not know which section you are presenting until 1 hour before lab, therefore you should learn all 4 parts.

For the final written lab report, each person must write his or her own (including figure legends), though you may confer about the content. The figures may be shared resources since they were produced as a team. Put your name as the first author and your labmates' names as coauthors.

Grading will be based on these grading rubrics.

Date Topics Lab Manual
Word Files
Jan
16 - 17

Lab orientation & pipetting

all needed lab files for week #1

Jan
23 - 24

Design first experiment

Graph in Excel (not Google)

Before Lab...
1) answer 4 Questions

During Lab...
2) use Synergy plate reader and Excel

all needed lab files for week #2

Jan
30 - 31

Day before lab: assemble oligos into promoter
(1 person/lab group)

Day of lab: perform GGA,  transform cells, plate cells

Before Lab...
1) answer 4 Questions
2) one person from each lab group comes to Wall 104, 4:30 Monday, to start assembling a promoter

During Lab...
3) Start GGA
4) GGA presentation
5) paper GGA activity
6) generate GGA PPT file
7) transform and plate cells

all needed lab files for week #3

Feb
6 - 7

Day before lab: start cells growing overnight

Day of lab: analyze cell cultures & generate graphs

Before Lab...
1) answer 4 Questions
2) one person from each lab group comes to Wall 104, 4:30 Monday, to start cells growing overnight

During Lab...
3) PCR to confirm promoter cloning
4) freeze each clone
5) measure promoter phenotype & graph

all needed lab files for week #4
Feb
13 - 14

Complete plasmid genotyping, work on oral presentations 

Before Lab...
1) Answer 4 Questions
2) Make sure you understand GGA
Overview and Detailed explanations

During Lab...
3) Run gel electrophoresis for plasmid genotyping
4) Hear detailed presention about GGA
5) Make PPT for oral presentation (due in 2 weeks)

all needed lab files for week #5
Feb
20 - 21

Prepare oral presentations

Oral Report Grading Rubric

Before Lab...
1) organize all your lab data

During Lab...
2) prepare oral presentation (iBOP Bingo)
3) draft research proposal for promoter v2 using this Research Proposal Template (graded)

all needed lab files for week #6
Feb
27 - 28

Give oral presentations

Oral Report Grading Rubric

Before Lab...
1) practice oral presentation; asignments emailed 1 hour before

During Lab...
2) give oral presentation, read peer comments
(iBOP Bingo)
3) submit your research proposal for promoter v2 using this Research Proposal Template (graded)

all needed lab files for week #7
Mar
5 - 6
Spring Break

No Lab Meeting

No Lab Manual for the holiday, you're on your own!
Mar
12 - 13

Day before lab: assemble oligos into promoter v2
(1 person/lab group)

Day of lab: perform GGA,  transform cells, plate cells

Before Lab...
1) answer 4 Questions
2) 1 person from each lab group comes to Wall 104, 4:30 Monday, to start assembling v2 promoter

During Lab...
3) Start GGA
4) refine GGA PPT file
5) transform and plate cells

all needed lab files for week #8
Mar
19 - 20

Day before lab: start cells growing overnight

Day of lab: miniprep plasmids; analyze cell cultures & generate graphs

Before Lab...
1) answer 4 Questions
2) 1 person from each lab group comes to Wall 104, 4:30 Monday, to start cells growing overnight

During Lab...
3) measure v2 promoter phenotype & graph
4) miniprep plasmid DNA

all needed lab files for week #9
Mar
26 - 27

Send DNA for sequencing

Before Lab...
1) Answer 4 Questions

During Lab...
2) Nanodrop DNA
3) Mix sequencing primers with diluted DNA template

all needed lab files for week #10
Apr
2 - 3
Analyze DNA Sequencing Results

Before Lab...
1) Answer 4 Questions

During Lab...
2) Align expected DNA sequences with experimental results
3) Generate graphics for your final report

all needed lab files for week #11
Apr
9 - 10

Prepare final lab preport (individually)

Lab Report Template

No Lab Meeting

Start working on final lab report
Apr
16 - 17

Submit Final Lab Report

Lab Report Template

Before Lab...
1) Work on your lab report

During Lab...
2) finish lab report
3) email individualized final lab report

Spend lab time wisely

Apr
23 - 24

Fellowships for you &
Course Evaluations

1) Fellowships for you (free money)
2) Course Evaluations

Please submit final course evaluations

Dr. Campbell's Bio113 Page


Copyright 2024, A. Malcolm Campbell
Send comments, questions, and suggestions to: macampbell@davidson.edu