Learned how to work with yeast with assistance of Robin Wright
(Univ. Washington Zoology Dept., yeast researcher, UW Teacher of the Year 1999 - 2000)
Used Bio101 total RNA isolation kit and protocol developed by Karen Bernd
(Davidson College, ASCB member, see her poster on Sunday)
Used Genisphere's 3DNA Submicro EX Expression Array Detection Kit
Three Primary Goals:
1) Isolate total RNA without inducing stress genes (RNAlater by Ambion)
2) Developed Quality Control steps to assess cDNA production (PUI-friendly)
3) Get usable data.
© Copyright 2000 Department of Biology, Davidson College, Davidson, NC 28036
Send comments, questions, and suggestions to: macampbell@davidson.edu