What have we done and what's next?


Dr. Karmella Haynes and Samantha Simpson ('09) Continued Flipping


We constructed 2 stacks of one pancake
(promoter flavored)

It flips!!




We constructed 2 stacks of one pancake
(gene flavored)

It flips, too!!



We Built A Lot of Parts!


Unexpected Problems = opportunities to learn more


Position Matters for RBS



It Flips Too Fast!



Needed Two Plasmids to Reduce Hin Production; Remove RE

Pancakes in pSB1A7
Hin in pSB3K3




Read Through Transcription

Tetracycline Resistance Takes Very Few Molecules



Built Insulated Plasmid



Convert Constructs to Numerical Symbols


Biological Equivalents

(1) , (2) = (-2) , (-1)



Distinguish 1,2 from -2, -1



pBad is not good promoter



What Are We Working On Right Now?

Model predicts behavior of all 8 constructs.
Mouse over this figure.



pLac Promoter and Tetr Reveal Secrets

White Colonies Live on Tet Plates
Faint Pink Colonies Live on Tet Plates
Cells Die
Red Colonies Live on Tet Plates




What have we gained, besides having fun and learning a lot?

Cross-trained students in biology and math/CS

Understand: Promoters, Reporter Strengths, Critical Parts, and System Behavior

Near completion of biological computer

Produce better transgenic organisms?

Biological digital data storage (0's and 1's) device?

Massive computation as cheap as an undercooked hamburger!


Biology Department

© Copyright 2007 Department of Biology, Davidson College, Davidson, NC 28036
Send comments, questions, and suggestions to: macampbell@davidson.edu