Spotted Scanning Standards (S3)
Emily Oldham and Dan Pierce ('03)
Experimental Design
Need Stronger Signal
3DNA Dendrimer Advantage = 300+ dyes per binding
DNA Concentrations Printed
Establish Scanning Parameters
Concentrations Alters Ratios
Sequence-Specific Variations
Altered 3DNA Sticky Ends
Clean Up Printed DNA: Clone PCR Products
Plasmids Improve Signal
Microarrays Underestimate Fold Change
© Copyright 2004 Department of Biology, Davidson College,
Davidson, NC 28036
Send comments, questions, and suggestions to: macampbell@davidson.edu
