Proteomics by 2D Gels
Two-dimensional Gel Electrophoresis
![](proteins/2Ddemo.jpg)
Cut out Spots and Identify Proteins by Tandom Mass Spectroscopy
Each peptide could be broken into many possible smaller fragments
![](proteins/slide7.gif)
![](proteins/slide10.gif)
Each ion will have its own mass to charge ratio
![](proteins/slide11.gif)
The real spectrum is compared to all possible spectra for a given species and the peptide sequence is identified
![](proteins/slide12.gif)
(animation
and images courtesy Stan Fields)
Overview Page
Genomics Course Page
Biology Department Main Page
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or (704) 894 - 2692
© Copyright 2002 Department of Biology, Davidson College,
Davidson, NC 28035