Isotope-Coded Affinity Tags (ICAT) Methodology

ICAT Methodology

Isolate two populations of proteins

Tag each population with different ICAT reagents: ICAT = Isotope-Coded Affinity Tags with mass differences of 8

light version with hydrogens

Heavy version with deuteriums

Pool ICAT-tagged protein populations

Cut proteins into smaller peptides

Purify ICAT-tagged peptides

Use MS/MS to quantify and identify ICAT pairs of peptides

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