Methodology for Functional Genomics

Step 1. Create a microarray of every gene in yeast genome.

Step 2. Isolate mRNA from yeast grown under two different conditions; generate labeled cDNA of two different colors.

Step 3. Mix the two probes and incubate with microarray.

Step 4. Wash and detect fluorescence.

Determine which genes are transcribed in each condition....

budding

non-budding

or both conditions...

budding and non-budding

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