Source	Green Cy3 Tiff File	Red Cy5 Tiff File
Long Exposure	MLL2828_L_w595.tif	MLL2828_L_w685.tif
Short Exposure	MLL2828_w595.tif	MLL2828_w685.tif

 

• Emily Oldham's ('03) Honors research on Microarray sources of variation (500 kb powerpoint file)
  (First place, 2003 North Carolina Academy of Sciences - collegiate division)
  
• Dan Pierce's ('03) research on improving and quantifying signal variation (1.2 MB powerpoint file)
  (Third place, 2003 North Carolina Academy of Sciences - collegiate division)
  

Pomona College, CA (Dr. Laura Hoopes, faculty advisor)

• 12 students working in 5 groups examined DNA replication mutant v. wild-type
• data available on Stanford Microarray Database
• Genetic Regulation in Eukaryotes Course Page

Muhlenberg College, PA (Dr. Denise Wallack, faculty advisor)

• 10 students examined the following conditions:
• (filled in soon)
• data available on Stanford Microarray Database
• link to course page www.muhlenberg.edu/depts/biology/courses/genome/

Swarthmore College, PA (Dr. Liz Vallen, faculty advisor)

• 6 students examined DNA replication mutant v. wild-type
• data available on Stanford Microarray Database
• link to research page http://www.swarthmore.edu/NatSci/Biology/bio_professors/vallen/projects.html

Mount Saint Mary's College, MD (Dr. Myra Derbyshire, faculty advisor)

• 8 students examined mutants that affect chromatin structure v. wild-type
• results presented at the Pennsylvannia Academy of Science meeting in April, 20001

  Microarray expression analysis of a Saccharomyces cerevisiae hst3 hst4 double mutant
  

  Dr. Myra K. Derbyshire ¹, Daniel J. Koppers¹, Marlon G. Lawrence¹, Heather L. Offutt¹, Loretta M. Rathvon¹, Harriete L. Sanderson¹, Nabil A., Shafi¹, Michael F. Smith¹, Megan E. Walsh¹, Dr. Jeffery Strathern^{2
  1}Genetics class. Mount Saint Mary's College, Emmitsburg, Maryland.
  ²NCI-Frederick Cancer Research and Development Center, Frederick, Maryland.
  

  The Saccharomyces cerevisiae SIR2 family of genes consists of SIR2 and four SIR2-related genes (HST1- HST4). The SIR2 gene product is a key regulator of gene expression believed to help form modified chromatin structures on the genes it regulates. SIR2 is important for the structure of the nucleolus, for repressing gene expression near chromosome telomeres and at special sequences involved in cell-type differentiation. HST1-HST4 gene products may modify chromatin structure at different chromosomal locations from those regulated by SIR2. S. cerevisiae hst3 hst4 double mutants have low viability, defective telomeric silencing, and altered
  cell-cycle progression. A hst3::kanMX4 (Saccharomyces Deletion Research Project, #11801) was crossed to a hst4::kanMX4 strain (#3350). The gene expression patterns of two meiotic segregants (HST3 HST4, hst3 hst4) were compared. Microarray technology was made possible through affiliation with GCAT (Genome Consortium for Active Teaching).
  

California Lutheran University, CA (Dr. Denis Revie, faculty advisor)

• 6 students examined anaerobic v. aerobic, and three concentrations of peroxide
• data available on Stanford Microarray Database
• link to course page http://public.clunet.edu/~revie/recombinant.htm

8 students examined growth conditions and harsh growth conditions
data available on Stanford Microarray Database
link to research page http://srv2.lycoming.edu/~newman/research/research.htm

students examined effects of drug treatments
data available on Stanford Microarray Database
link to research page http://csm.uscolo.edu/biology/dcaprio.html

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GCAT members thank Drs. Pat Brown, David Botstein, Barbara Dunn, and the other members of the Stanford Microarray Database for their generosity in allowing undergraduate data to be included in their database. We also thank Pat Brown and members of his lab for producing the yeast microarrays for our use. Without this support, GCAT would not have been so successful in its first year of existence.