NADP+-dependent isocitrate dehydrogenase (IDH)

To the left is shown the 3D structure of NADP-dependent isocitrate dehydrogenase IDH from Escherichia coli, complexed with isocitrate dehydrogenase, Ca2+ and NADP.

Isocitrate dehydrogenase is one of the enzymes involved in the Kreb's cycle. It catalyses the oxidation of isocitrate to alpha-ketoglutarate, with NADP acting as a coenzyme (in the case of E.coli -- eukaryotes have a NAD dependent form of the enzyme as well). The reaction is facilitated by a divalent metallic cofactor (Mg2+, Mn2+, etc.)
SRS entry for IDH



 Show NADP (coenzyme - H acceptor)

 Show isocitrate and calcium (substrate+metallic cofactor)

 Show phosphorylation site (Serine 113)
      Phosphorylation at this site is used to regulate the activity of E.coli IDH. (Pubmed)

 Show isocitrate and isopropylmalate
     dehydrogenase family signature
     (residues 303-322, Prosite)

 Show DAB match with IDH3 (NAD+ dependent)
     (residues 221-240)

 Show DAB match with HSPs
     (residues 221-240)

Comments? Questions? Please contact rakarnik@davidson.edu.