Nicole Hesson's Molecular Biology Web Page
This web page was produced as an assignment for an undergraduate course at Davidson College.
The goal of this
semester’s lab is to clone all five IDH genes from Saccharomyces cerevisiae
using PCR and express the encoded proteins in bacteria. After using the yeast gene as a probe to see
if it hybridizes any orthologs from yeast, we will perform enzyme assays to
determine if the genes retain their properties when expressed in a
prokaryote. The experiment is described in more detail on the weekly protocol page
Figure 1: Wildtype W303 cells with GFP imported in mitochondria and DNA stained with dapi.
Image provided by Teresa Rinaldi
Project 1: Describe
the five IDH Genes.
IDH1
Project 2: Structure and Orthologs
Project 3: Paper Review
Fantastic Resources for Yeast Information
References
Molecular Biology (BIO 304), 2002. "Spring 2002 Lab Project Explained." bio.davidson.edu/Courses/Molbio/labcontext.html
Purves, W.K., et al. 1998. Life: The Science of Biology, 5th Ed. Sinauer Associates, MA.
Questions, Comments or Concerns?