Gene Networks Database

    LOCUS   Rr_SUSMSP       868 bp    mRNA            INV       10-JUN-1999
DEFINITION  Sea urchin (S.purpuratus) mesenchyme-specific cell surface
            glycoprotein, regulatory region (a region between exons 6 and 7)
DBSOURCE    
KEYWORDS    cell surface glycoprotein.
SOURCE      Sea urchin
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryota; Metazoa; Echinodermata; Echinozoa; Echinoidea;
            Euechinoidea; Echinacea; Echinoida; Strongylocentrotidae;
            Strongylocentrotus.
REFERENCE   1  (bases 1..868)
  AUTHORS   Raman, V., Andrews, M.E., Harkey, M.A. and Raff, R.A.
  TITLE     Protein-DNA interactions at putative regulatory regions of two
            coordinately expressed genes, msp130 and PM27, during 
            sceletodenesis in sea urchin embryos
  JOURNAL   Int. J. Dev. Biol. 37, 499-507 (1993)
  MEDLINE   87191419
COMMENT     Comparison of the PM27 promoter to SM50 promoter and 850-bp 
            region of msp130 gene located between exons 6 and 7 revealed 
            three larger sequence elements (designated A, B and C) that are 
            strictly similar in these genes. A and B elements bind proteins 
            on gastrula and pluteus stages, while no binding was observed 
            with morula extracts. Competitive gel shift assay showed that A 
            and B elements of PM27 and msp130 genes bind the same proteins.
            The A and C elements identified in this work seem to be not identical to A and C
            elements in the SM50 regulatory region, which are
            identified by Makabe et al (1995).
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Strongylocentrotus purpuratus"
     element A       96..123
                     /evidence=sequence inspection
                     /evidence=DNase I footprinting
                     /evidence=gel shift assays
                     /citation=[1]
     misc_feature    228..239
                     /note="P7I"    
                     /evidence=sequence inspection 
                     /citation=[1]
     exon 6a         347..416
                     /citation=[1]
     element C       433..446
                     /evidence=sequence inspection
                     /citation=[1]
     misc_feature    453..457
                     /site="CCAAT"
                     /evidence=sequence inspection
                     /citation=[1]
     misc_feature    549..556
                     /site="P3A"
                     /evidence=sequence inspection
                     /citation=[1]
     misc_feature    781..785 
                     /site="CCAAT"
                     /evidence=sequence inspection
                     /citation=[1]
     element B       781..797  
                     /evidence=sequence inspection
                     /evidence=DNase I footprinting
                     /evidence=gel shift assays
                     /citation=[1]
ORIGIN      
       1  aagcttgttt gtaggcccca caggtaataa aatattgaaa gtctaacaga atctttggaa
      61  cgaacaagtg tcagttgaaa aagagtaagt agataagccg catttacgtg tgcattgggt
     121  gtaaataact aatcagtata taaaatcaaa gaggatatca atactaatca ataaagtatg
     181  gcaaatgcaa agaaaagcat gaaatatcaa tatgacgtta ataatcgttt gttcgtataa
     241  tcgacgagat gttaactgaa tatactaaaa acgaccttag agggtctaat aaaacataga
     301  caccatgcat taaatcgtat ttgttttctt cttatttttc ccttctagag ctacttcccc
     361  aacccgtgat agacagtccc ctgggacaag caatggctga agagacacag ttaggtgagt
     421  ttgtattgaa gtctgaaatt caaaacatgt gtattggccg cattttttta tatgccatac
     481  aggtaaaatc tatcttaaca acacaccctg gcctcattaa ctactcaata cacaatgatc
     541  caagcgttca tgcgctacca tatattcaat gcaaacacat tgctgaggat aagtgtcaaa
     601  caatctcatc acaaaagttg agatttctta agaatgtctg cgagccattt atgagggcgg
     661  ttttgtacca tacattttaa aaaagtaaga aaatatatgt aaataaattg tgctggtagt
     721  tcataatcat ttcaaacaaa caaaaatctc gccctatcaa atggggaatt ctaaatatct
     781  attggcccaa actttgatga gtaagtcctt tttcttctat ttccattcga taaccttaag
     841  acctgatgtt gcactttgaa ttaagctt