The data presented here were extracted with permission from Ruedi Aebersonld from Gygi et al., 1999. Nature Biotechnology 17: 994 - 999. This is the first paper which described the ICAT method.
Figure 1. Structures of the ICAT reagents with Heavy on top (with deuterium)
and Light on bottom (with plain Hydrogen)
Figure 2. ICAT strategy for quantifying and identifying different protein pairs.
Figure 3. ICAT quantification analysis of a protein mixture in Table 1.
Figure 4. ICAT sequence identification of a pair of peptides from a light and heavy pair.
Figure 5. ICAT ananlysis of alcohol dehydrogenase isozymes in yeast.
This was a part of the glucose v. galactose experiments mention in Table 2.
© Copyright 2001 Department of Biology, Davidson College, Davidson, NC 28036
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