LpC2 gene codes for the cytosceletal type actin (Fang et al., 1996). Actin represents one of the most abundunt and best characterized structural
components in eucaryotic cells, involved in a wide variety of cytoskeletal, locomotive, and tension-generating functions (Davidson, 1989).
Each haploid L. pictus genome contains a single copy of each of the actin genes LpC1, LpC2, LpC3, LpC4, and LpM.
Southern blot analysis of genomic DNA clones have detected no close linkage of L.pictus actin genes in contrast to
the cytosceletal actins of S. purpuratus, which are clustered into two linkage groups of related genes (Fang et al., 1996).
Protein
LpC2 protein belongs to a family of the cytoskeletal
actins (Fang et al., 1996).
SWISS_PROT: P53466
Subcellular location
Cytoplasmic
Expression Pattern
After LpC1 mRNA, the LpC2 mRNA is the most abundant
actin mRNA expressed during embryogenesis.
The LpC2 transcripts are first detected in the mesenchyme blastula and accumulate in late
gastrula embryos.
The LpC2 probe hybridized predominantly to transcripts of 2,1 and 2.2 kb.
Whole mount in situ hybridization indicate the LpC2 actin gene is intensively
expressed in the aboral ectoderm (AE) at the gastrula and pluteus stages.
Scattered mesenchyme cells are lightly stained in late gastrula and prism embryos.
Some endodermal cells confined to the stomach and intestine of pluteus embryos are
lightly stained as well (Fang et al., 1996).
mRNA level
Temporal accumulation
Method: Nothern blot hybridization
Reference: Fang et al., 1996
Stage
Egg
Hatching blastula
Mesenchyme blastula
Late gastrula
Prism
Pluteus
Level
-
-
+
+
+
+
+
Spatial localization
Method: Whole mount in situ hybridization
Reference: Fang et al., 1996
Stage
Gastrula
Prism
Late pluteus
Tissue
AE, scattered mesenchyme cells
AE, scattered mesenchyme cells
AE, endoderm cells confined to stomach and intestine
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