Molecular Biology

bio.davidson.edu/Biology/Courses/Molbio/molecular.html

Semester-Long Module

• Clone Yeast IDH gene via PCR
• Perform "Zooblot" with Yeast Gene as Probe
• Express Yeast IDH in E. coli
• Perfrom Western Blot on Expressed IDH

Students' Southern Blot using Yeast IDH as Probe
ECL detection of IDH from Several Species (L to R: MW markers, E. coli , yeast, Chlamydomonas, Fly, Mouse, Human)

Students' Western Blot using Epitope Tag
Detection of Yeast IDH expressed in E. coli

Assay Measuring Recombinant Yeast IDH Activity
Production of NADPH was compared between pig heart IDH (Sigma #I-2002; blue squares), recombinant yeast IDH expressed in E. coli (black squares), and the yeast IDH gene cloned in reverse orientation (red circles). For a more complete description of the method, see either Erin Mooney's thesis or the Bio111 lab manual.

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