This web page was produced as an assignment for an undergraduate course at Davidson College
Spike Orthologs:
Avian Infectious Bronchitis Coronavirus Spike Protein
Website Navigation:
Spike Protein Structure and Function
More Information on SARS and the Spike Protein:
Centers for Disease Control and Prevention
Other Interesting Viral Proteins:
Webpage created by Kevin Saunders, an undergraduate student at Davidson College.
The Avian Infectious Bronchitis Virus (AIVB) Spike Protein is very similar in structure and function to the SARS-CoV Spike protein (Casais et al., 2003; Rota et al., 2003). The AIVB Spike protein consists of four domains: a signal sequence, the ectodomain, the transmembrane domain, and the cytoplasmic tail (Casais et al., 2003). The protein is cleaved into subunits, S1 and S2, which are approximately 535 amino acids and 627 amino acids in length respectively. The subunits are responsible for binding to the target receptor and membrane fusion. The S2 subunit contains the heptad repeat regions common spike proteins (Casais et al., 2003). |
This ortholog has 9 subsequences that contain positive matches for the SARS-CoV S protein. These subsequence total 558 amino acids of the total 1165 amino acids in the Avian infectious bronchitis coronavirus Spike Protein. Fifty-three percent of this 558 amino acids have positive matches to the SARS-CoV amino acid sequence. |
The Amino Acid Sequence of the Avian infectious bronchitis coronavirus Spike Protein |
1 MLGKSLFLVT ILCALCSANL FDSANNYVYY YQNAFRPPNG WHLQGGAYAV VNSTNYTNNA 61 GSVNECTIGV IKDVYNYSAA AIAMTAPLQG MAWSKSQFCS AHCNFSEITV FVTHCYSSGS 121 GSCPITGMIP QGHIRISAMK NGSLFYNLTV SVSKYPNFKS FQCVNNFTSV YLNGDLVFTS 181 NATTGVKSAG VYFKAGGPVN YNIMKEFKVL AYFVNGTVQD VILCDDTPRG LLACQYNNGN 241 FSDGFYPFTN SSLVKEKFIV YRENSVNTTL TLTNYTFYNV ANASPNRGGV QSIPTYQTQT 301 AQSGYYNFNL SFLSSFVYKE SNYMYGSYHP ACNFRLETIN NGLWFNSLSV SLAYGPFQGG 361 CKQSVFSSRA TCCYAYSYNG PRACKGVYAG ELLQNFECGL LVYVTKSDGS RIQTATVPPV 421 VTQHNYNNIT LNTCVDYNIY GRVGRGFITN VTDSSSSYNY LADAGLAILD TSGAIDIFVV 481 QGEHGLNYYK VNPCEDVNHQ FVVSGGKLVG ILTSRNATGS QPLENQFYIK LTKETRRFRR 541 STSDNVTSCP YVTYGRFCIQ PDGSIKQIVP QELENFVAPL LNVTENVLIP SSFNLTVTDE 601 YIQTRMDKVQ INCLQYVCGN SLDCRKLFQQ YGPVCDNILS IVNSVGQKED MELLTFYSST 661 KPAGYNAPVF SNISTGDFNI SLLLTPPSSP SGRSFIEDLL FTSVETVGLP TDAEYKKCTA 721 GPLGTLKDLI CAREYNGLLV LPPIITADMQ TMYTASLVGA MAFGGITAAG AIPFATQIQA 781 RINHLGITQS LLLKNQEKIA ASFNKAIGHM QEGFRSTSLA LQQVQDVVNK QSAILTETMN 841 SLNKNFGAIS SVIQDIYAQL DAIQADAQVD RLITGRLSSL SVLASAKQSE YIRVSQQREL 901 ATQKINECVK SQSNRYGFCG SGRHVLSIPQ NAPNGIVFIH FTYTPQSFVN VTAIVGFCVQ 961 PANASQYAIV PVNGRGIFIQ VNGSYYITAR DMYMPRDITA GDIVTLTSCQ ANYVNVNKTV 1021 ITTFVEDDDF DFDDELSKWW NDTKHELPDF DDFNYTVPIL NISGEIDRIQ GVIQGLNDSL 1081 IDLEELSIIK TYIKWPWYVW LAIGFAVIIF ILILGWVFFM TGCCGCCCGC FGIIPLMSKC 1141 GKKSSYYTTF DNDVVTEQYR PKKSV |
| Sequence taken from Liu et al., 2005. |
Orthologs
Canine coronavirus Spike Protein
Murine Hepatitis Virus Spike Protein
Avian infectious bronchitis coronavirus Spike Protein
Works Cited
Casais, R., Dove, B., Cavanagh, D., and Britton, P. 2003. Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Demonstrates that the Spike Protein Is a Determinant of Cell Tropism. Journal of Virology 77(16): 9084-9089.
Liu, S., Chen, J., Chen, J., Kong, X., Shao,Y., Han, Z., Feng, L., Cai, X., Gu, S. and Liu, M. 2005. Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas) J. Gen. Virol. 86 (3): 719-725.
Rota, P. et al. 2003. Characterisation of a novel Coronavirus associated with Severe Acute Respiratory Syndrome. Science 300: 1394-1399.