This web page was produced as an assignment for an undergraduate course at Davidson College
Spike Orthologs:
Canine Coronavirus Spike Protein
Website Navigation:
Spike Protein Structure and Function
More Information on SARS and the Spike Protein:
Centers for Disease Control and Prevention
Other Interesting Viral Proteins:
Webpage created by Kevin Saunders, an undergraduate student at Davidson College.
The Spike protein of the Canine Respiratory Coronavirus (CRCV) has several characteristics common to Spike proteins of coronaviruses (Wesseling et al., 1994). There is a subset of hydrophobic amino acids at the amino terminus of the CRCV S protein. The carboxyl terminus of this spike protein also has a hydrophobic region, which serves as the membrane anchor. Lastly, there are heptad repeats preceeding the membrane anchor region. The CRCV Spike protein differs from the SARS-CoV Spike protein in that it is not cleaved into two subunits. The CRCV Spike protein lacks the proteolytic cleavage site necessary for cleavage into two subunits (Wesseling et al., 1994). |
| This ortholog has 13 subsequences that match the SARS-CoV S protein amino acid sequence. These subsequences equal 759 amino acids; of which 404 (53 percent) amino acids are positive matches to the SARS-CoV S protein amino acid sequence. |
The Amino Acid Sequence of the Canine Respiratory Coronavirus Spike Protein |
1 MFLILLISLP MAFAVIGDLK CTTVSINDVD TGAPSISTDV VDVTNGLGTY YVLDRVYLNT 61 TLLLNGYYPT SGSTYRNMAL KGTLLLSTLW FKPPFLSDFI DGVFAKVKNT KVIKDGVVYS 121 EFPAITIGST FVNTSYSVVV QPHTTNLDNK LQGLLEISVC QYTMCDYPHT MCHPNLGNKR 181 IELWHWDTGV VPCLYKRNFT YDVNADYLYS HFYQEGGTFY AYFTDTGVVT KFLFHVYLGT 241 VLSHYYVMPL TCNSAMTLEY WVTPLTFKQY LLAFNQDGVI FNAVDCKSDF MSEIKCKTLS 301 IAPSTGVYEL NGYTVQPIAD VYRRIPNLPD CNIEAWLNDK SVPSPLNWER KTFSNCNFNM 361 SSLMSFIQAD SFTCNNIDAA KIYGMCFFSI TIDKFAIPNG RKVDLQMGNL GYLQSFNYRI 421 DTTATSCQLY YNLPASNVSI SRFNPSIWNR RFGFTEQSVF KPQPVGVFTD HDVVYAQHCF 481 KAPTNFCPCK LNGSLCVGSG FGIDAGYKNS GIGTCPAGTN YLTCYNANQC DCLCTPDPIL 541 SKSTGPYKCP QTKYLVGIGE HCSGLAIKSD YCGGNPCTCQ PKAFLGWSVD SCLQGDRCNI 601 FANFILHGVN SGTTCSTDLQ KSNTDIILGV CVNYDLYGIT GQGIFVEVNA TYYNSWQNLL 661 YDSNGNLYGF RDYLTNRTFM IRSCYSGRVS AGFHSNSSEP ALLFRNIKCN YVFNNTLSRQ 721 LQPINYFDSY LGCVVNADNS TSSSVQTCDL TVGSGYWGDY STQRRSRRTI TTGYRFTNFE 781 PFTVNPVNDS LHPVGGLYEI QIPSEFTIGN MEEFIQTRSP KVTIDCPVFV CGDYAACKSQ 841 LVEYGSFCDN INAILTEVNE LLDTTQLQVA NSLMNGVTLS TKLKDGFNFN VDDINFSPVL 901 GCLGSECNKV SSRSAIEDLL FSKVKLSDVG FVDAYNNCTG GAEIRDLICV QSYNGIKVLP 961 PLLSENQISG YTLAATFASL FPPWSAAAGV PFYLNVQYRI NGIGVTMDVL TQNQKLISNA 1021 FNNALDAIQE GFDATNSALV KIQAVVNANA EALNNLLQQL SNKFGAISAS LQEILSRLDA 1081 LEAQAQIDRL INGRLTALNA YVSQQLSDST LVKFSAAQAM EKVNECVKSQ SSRINFCGNG 1141 NHIISLVQNA PYGLYFIHFS YVPTKYVTAK VSPGLCIAGD RGIAPKSGYF VNVNNTWMFT 1201 GSGYYYPEPI TGNNVVVMST CAVNYTKAPD VMLNISTPNL PDFKEELDQW FKNQTLMAPD 1261 LSLDYINVTF LDLQDEMNRL QEAIKVLNHS YINLKDIGTY EYYVKWPWYV WLLIGLAGVA 1321 MLVLLFFICC CTGCGTSCFK KCGGCCDDYT GHQELVIKTS HDD |
| Sequence taken from Erles et al., 2003 |
Orthologs
Canine coronavirus Spike Protein
Murine Hepatitis Virus Spike Protein
Avian infectious bronchitis coronavirus Spike Protein
Works Cited
Erles, K., Toomey, C., Brooks, H., and Brownlie, J. 2003. Detection of a group 2 coronavirus in dogs with canine infectious respiratory disease. Virology 310(2):216-23.
Wesseling, J., Vennema, H., Godeke, G.J., Horzinek, M., Rottier, PJ. 1994. Nucleotide sequence and expression of the spike (S) gene of canine coronavirus and comparison with the S proteins of feline and porcine coronaviruses. J. Gen. Virol. 75 (7): 1789-94.