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Only For Faculty at American Institutions

2009 Workshop #1 (20 participants)

2009 Workshop #2 (24 participants)

2009 Instructors

Todd Eckdahl, Laurie Heyer, Anne Rosenwald, Consuelo Alvarez,
Charles Hauser, Malcolm Campbell, Edison Fowlks

2009 Binder Contents

Travel and Contact Information

Bringing a Laptop (optional)

Highlights:

Free Software and Training

Interdisciplinary Team Participation Encouraged

Produce RNA; cDNA probes; Hybe; Wash; Scan; Analyze

Yeast full-genome microarrays

Computers Provided but You Can Bring Laptops

We will use Genisphere’s 3DNA method instead of direct incorporation. We give each participants a GCAT Flash Drives (1 GB each) which will allow us to deliver the workshop materials and for participants to archive all their data. We will collect all the drives during the scanning period and return them with all the tiff files the next morning in time for dry lab.

Participants will prep their own RNA. We will provide for them frozen yeast pellets and the RNA isolation kits. They will analyze the quality of their RNA by spectrophotometer only (i.e., will not run gels) and decide if they want to use their RNA or some prepared by the instructors prior to the workshop. Participant RNA isolation will take place on Day 2 for both workshops during their half day of wet lab.

Selection Criteria

GCAT Protocols

Additional Online Resources

Purpose: Genome Consortium for Active Teaching (GCAT) offers hands-on workshops for faculty who conduct research with undergraduate students and/or teach undergraduate courses to learn about gene expression analysis via microarrays.

Workshop Organization:
Part 1 of the workshop begins with data analysis (see schedule below) to introduce the microarray method and will cover data analysis using public domain data from the literature and from GCAT. Participants will learn public domain and open source MAGIC Tool spot-finding and analysis software. The MIAME background information and its use in microarray evaluation will be covered. Participants will analyze data sets in short projects. Each of the two workshops has room for 20 participants.

Part 2 will be a hybridization workshop which will involve hands-on preparation of fluorescently labeled probes for yeast expression microarrays, their hybridization, data acquisition and data analysis using the methods presented in part 1 of the workshop.

Part 3 concludes with data analysis of the microarrays you produced. This will also serve to reinforce what you learned about data analysis.

Where: Morehouse College, Atlanta, GA

When: workshop #1: July 6 - 10 mid day; and workshop #2: 7 - 11, 2009

Costs and Scholarships: If funded by the NSF, this workshop will be completely free (housing, food, lab fees) for each participant with one catch. You pay for your airfare in advance but you will be reimbursed if you submit an action plan for implementing microarrays in your curriculum and submit receipts. For those with financial need for airfare in advance, you may petition to have this paid in advance.

Only For Faculty at American Institutions

Email Edison Fowlks or Malcolm Campbell with Questions

See photos from 2003 workshops

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See photos from 2008 workshops

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