Laboratory Manual

Principles of Biology I (Bio 111) - Cell and Molecular Biology

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1999 - 2000

Department of Biology - Davidson College

A	Oral Presentations	i
B	Writing an Abstract	iii
C	Guidelines for Scientific Papers	v
D	How to Use a "Pipetman" Take the First Lab Quiz	1
Lab #1	Introduction to Spectrophotometry	3
Lab #2	Isocitrate Dehydrogenase (IDH): Measurement of Enzyme Activity	11
Lab #3	Varying Parameters Affects IDH Activity Take the Second Lab Quiz	17
	ORAL PRESENTATIONS AND WRITTEN ABSTRACT
Lab #4	Using Microscopes Tutorial on Using Bio111 Microscopes	22
	A Series of Experiments on Chlamydomonas Mating	26
Lab #5	Chlamydomonas Flagellar Regeneration	31
Lab #6	Variations that Affect Flagellar Regeneration	36
Lab #7	A Beginner's Guide to Statistics and Excel Take the Third Lab Quiz	37
	PRESENTATIONS AND WRITTEN MATERIALS AND METHODS
Lab #8	Pouring Agar Plates	45
	Spot-Overlay Ames Test of YFPM (Your Favorite Potential Mutagen) Sodium Azide Mutagenesis Information and 3D structure	46
Lab #9	Quantitating a Mutagen Search NIEHS Data Base of Mutagens Take the Forth Lab Quiz	57
	WRITTEN FULL SCIENTIFIC PAPER
Lab #10	What is Your Genotype? A PCR Answer Movie of PCR Method Shockwave Movie of PCR Method (click here for shockwave plug-in)	60
Lab #11	PCR Results Take the Fifth and Final Lab Quiz	64

Appendix A - Cricket Graph

Appendix B - NIH Image to Measure Flagellar Length

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Fall Semester

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Spring Semester

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• Send email to Dr. John Williamson

© Copyright 2000 Department of Biology, Davidson College, Davidson, NC 28036
Send comments, questions, and suggestions to: macampbell@davidson.edu