Laboratory Manual

Principles of Biology I (Bio 111) - Cell and Molecular Biology

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1999 - 2000

Department of Biology - Davidson College


A Oral Presentations

i

B Writing an Abstract

iii

C Guidelines for Scientific Papers

 v

D

How to Use a "Pipetman"

Take the First Lab Quiz

1

Lab #1 Introduction to Spectrophotometry

3

Lab #2 Isocitrate Dehydrogenase (IDH): Measurement of Enzyme Activity

11

Lab #3

Varying Parameters Affects IDH Activity

Take the Second Lab Quiz

17

 

ORAL PRESENTATIONS AND WRITTEN ABSTRACT

 
Lab #4

Using Microscopes

Tutorial on Using Bio111 Microscopes

22

  A Series of Experiments on Chlamydomonas Mating

26

Lab #5 Chlamydomonas Flagellar Regeneration

31

Lab #6 Variations that Affect Flagellar Regeneration

36

Lab #7

A Beginner's Guide to Statistics and Excel

Take the Third Lab Quiz

37

 

 PRESENTATIONS AND WRITTEN MATERIALS AND METHODS

 
Lab #8 Pouring Agar Plates

45

Spot-Overlay Ames Test of YFPM (Your Favorite Potential Mutagen)

Sodium Azide Mutagenesis Information and 3D structure

46

Lab #9

Quantitating a Mutagen

Search NIEHS Data Base of Mutagens

Take the Forth Lab Quiz

57

 

 

WRITTEN FULL SCIENTIFIC PAPER

 
Lab #10

What is Your Genotype? A PCR Answer

Movie of PCR Method

Shockwave Movie of PCR Method
(click here for shockwave plug-in)

60

Lab #11

 

PCR Results

Take the Fifth and Final Lab Quiz

64

Appendix A - Cricket Graph

Appendix B - NIH Image to Measure Flagellar Length

 

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